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ROCK1 Protein (AA 2-1354) (His tag)

Crystallography grade ROCK1 Origine: Humain Hôte: Cellules d'insectes Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
N° du produit ABIN3095084
  • Antigène Voir toutes ROCK1 Protéines
    ROCK1 (rho-Associated, Coiled-Coil Containing Protein Kinase 1 (ROCK1))
    Type de proteíne
    Recombinant
    Attributs du protein
    AA 2-1354
    Origine
    • 4
    • 3
    • 1
    • 1
    Humain
    Source
    • 3
    • 2
    • 1
    • 1
    Cellules d'insectes
    Purification/Conjugué
    Cette ROCK1 protéine est marqué à la His tag.
    Application
    Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)
    Séquence
    STGDSFETRF EKMDNLLRDP KSEVNSDCLL DGLDALVYDL DFPALRKNKN IDNFLSRYKD TINKIRDLRM KAEDYEVVKV IGRGAFGEVQ LVRHKSTRKV YAMKLLSKFE MIKRSDSAFF WEERDIMAFA NSPWVVQLFY AFQDDRYLYM VMEYMPGGDL VNLMSNYDVP EKWARFYTAE VVLALDAIHS MGFIHRDVKP DNMLLDKSGH LKLADFGTCM KMNKEGMVRC DTAVGTPDYI SPEVLKSQGG DGYYGRECDW WSVGVFLYEM LVGDTPFYAD SLVGTYSKIM NHKNSLTFPD DNDISKEAKN LICAFLTDRE VRLGRNGVEE IKRHLFFKND QWAWETLRDT VAPVVPDLSS DIDTSNFDDL EEDKGEEETF PIPKAFVGNQ LPFVGFTYYS NRRYLSSANP NDNRTSSNAD KSLQESLQKT IYKLEEQLHN EMQLKDEMEQ KCRTSNIKLD KIMKELDEEG NQRRNLESTV SQIEKEKMLL QHRINEYQRK AEQENEKRRN VENEVSTLKD QLEDLKKVSQ NSQLANEKLS QLQKQLEEAN DLLRTESDTA VRLRKSHTEM SKSISQLESL NRELQERNRI LENSKSQTDK DYYQLQAILE AERRDRGHDS EMIGDLQARI TSLQEEVKHL KHNLEKVEGE RKEAQDMLNH SEKEKNNLEI DLNYKLKSLQ QRLEQEVNEH KVTKARLTDK HQSIEEAKSV AMCEMEKKLK EEREAREKAE NRVVQIEKQC SMLDVDLKQS QQKLEHLTGN KERMEDEVKN LTLQLEQESN KRLLLQNELK TQAFEADNLK GLEKQMKQEI NTLLEAKRLL EFELAQLTKQ YRGNEGQMRE LQDQLEAEQY FSTLYKTQVK ELKEEIEEKN RENLKKIQEL QNEKETLATQ LDLAETKAES EQLARGLLEE QYFELTQESK KAASRNRQEI TDKDHTVSRL EEANSMLTKD IEILRRENEE LTEKMKKAEE EYKLEKEEEI SNLKAAFEKN INTERTLKTQ AVNKLAEIMN RKDFKIDRKK ANTQDLRKKE KENRKLQLEL NQEREKFNQM VVKHQKELND MQAQLVEECA HRNELQMQLA SKESDIEQLR AKLLDLSDST SVASFPSADE TDGNLPESRI EGWLSVPNRG NIKRYGWKKQ YVVVSSKKIL FYNDEQDKEQ SNPSMVLDID KLFHVRPVTQ GDVYRAETEE IPKIFQILYA NEGECRKDVE MEPVQQAEKT NFQNHKGHEF IPTLYHFPAN CDACAKPLWH VFKPPPALEC RRCHVKCHRD HLDKKEDLIC PCKVSYDVTS ARDMLLLACS QDEQKKWVTH LVKKIPKNPP SGFVRASPRT LSTRSTANQS FRKVVKNTSG KTS
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Attributs du produit
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Human ROCK1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Purification
    Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Pureté
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Stérilité
    0.22 μm filtered
    niveau d'endotoxine
    Protein is endotoxin free.
    Classe de qualité
    Crystallography grade
    Top Product
    Discover our top product ROCK1 Protéine
  • Indications d'application
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    Commentaires

    In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Conseil sur la manipulation
    Avoid repeated freeze-thaw cycles.
    Stock
    -80 °C
    Stockage commentaire
    Store at -80°C.
    Date de péremption
    Unlimited (if stored properly)
  • Antigène
    ROCK1 (rho-Associated, Coiled-Coil Containing Protein Kinase 1 (ROCK1))
    Autre désignation
    ROCK1 (ROCK1 Produits)
    Synonymes
    LOC100229794 Protein, CG9774 Protein, DROK Protein, DRhk Protein, DRok Protein, Dmel\\CG9774 Protein, Drok Protein, ROCK Protein, ROCK-1 Protein, ROCK1 Protein, ROK Protein, ROKalpha/beta Protein, Rhk Protein, RhoK Protein, Rock Protein, Rok Protein, dROK Protein, dRok Protein, drok Protein, 1110055K06Rik Protein, Rock-I Protein, P160Rock Protein, ROCK-I Protein, P160ROCK Protein, p160ROCK Protein, Rho associated coiled-coil containing protein kinase 1 Protein, Rho kinase Protein, Rho-associated coiled-coil containing protein kinase 1 Protein, Rho-associated, coiled-coil containing protein kinase 1 Protein, ROCK1 Protein, Rok Protein, Rock1 Protein, rock1 Protein
    Sujet
    Protein kinase which is a key regulator of actin cytoskeleton and cell polarity. Involved in regulation of smooth muscle contraction, actin cytoskeleton organization, stress fiber and focal adhesion formation, neurite retraction, cell adhesion and motility via phosphorylation of DAPK3, GFAP, LIMK1, LIMK2, MYL9/MLC2, PFN1 and PPP1R12A. Phosphorylates FHOD1 and acts synergistically with it to promote SRC-dependent non-apoptotic plasma membrane blebbing. Phosphorylates JIP3 and regulates the recruitment of JNK to JIP3 upon UVB-induced stress. Acts as a suppressor of inflammatory cell migration by regulating PTEN phosphorylation and stability. Acts as a negative regulator of VEGF-induced angiogenic endothelial cell activation. Required for centrosome positioning and centrosome-dependent exit from mitosis. Plays a role in terminal erythroid differentiation. May regulate closure of the eyelids and ventral body wall by inducing the assembly of actomyosin bundles. Promotes keratinocyte terminal differentiation. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization. {ECO:0000269|PubMed:10436159, ECO:0000269|PubMed:10652353, ECO:0000269|PubMed:11018042, ECO:0000269|PubMed:11283607, ECO:0000269|PubMed:17158456, ECO:0000269|PubMed:18573880, ECO:0000269|PubMed:18694941, ECO:0000269|PubMed:19036714, ECO:0000269|PubMed:19131646, ECO:0000269|PubMed:19181962, ECO:0000269|PubMed:19997641, ECO:0000269|PubMed:21072057, ECO:0000269|PubMed:8617235, ECO:0000269|PubMed:9722579}.
    Poids moléculaire
    159.0 kDa Including tag.
    UniProt
    Q13464
    Pathways
    Dynamique des Microtubules, Signalisation WNT, M Phase, Maintenance of Protein Location, Signaling Events mediated by VEGFR1 and VEGFR2, Thromboxane A2 Receptor Signaling
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