SASH1 Protein (AA 1-1247) (Strep Tag)

N° du produit ABIN3095229

Détail du produit

Antigène: SASH1 (SAM and SH3 Domain Containing 1 (SASH1))

Type de proteíne: Recombinant

Attributs du protein: AA 1-1247

Origine: Humain

Source: Tobacco (Nicotiana tabacum)

Purification/Conjugué: Cette SASH1 protéine est marqué à la Strep Tag.

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Séquence: MEDAGAAGPG PEPEPEPEPE PEPAPEPEPE PKPGAGTSEA FSRLWTDVMG ILDGSLGNID DLAQQYADYY NTCFSDVCER MEELRKRRVS QDLEVEKPDA SPTSLQLRSQ IEESLGFCSA VSTPEVERKN PLHKSNSEDS SVGKGDWKKK NKYFWQNFRK NQKGIMRQTS KGEDVGYVAS EITMSDEERI QLMMMVKEKM ITIEEALARL KEYEAQHRQS AALDPADWPD GSYPTFDGSS NCNSREQSDD ETEESVKFKR LHKLVNSTRR VRKKLIRVEE MKKPSTEGGE EHVFENSPVL DERSALYSGV HKKPLFFDGS PEKPPEDDSD SLTTSPSSSS LDTWGAGRKL VKTFSKGESR GLIKPPKKMG TFFSYPEEEK AQKVSRSLTE GEMKKGLGSL SHGRTCSFGG FDLTNRSLHV GSNNSDPMGK EGDFVYKEVI KSPTASRISL GKKVKSVKET MRKRMSKKYS SSVSEQDSGL DGMPGSPPPS QPDPEHLDKP KLKAGGSVES LRSSLSGQSS MSGQTVSTTD SSTSNRESVK SEDGDDEEPP YRGPFCGRAR VHTDFTPSPY DTDSLKLKKG DIIDIISKPP MGTWMGLLNN KVGTFKFIYV DVLSEDEEKP KRPTRRRRKG RPPQPKSVED LLDRINLKEH MPTFLFNGYE DLDTFKLLEE EDLDELNIRD PEHRAVLLTA VELLQEYDSN SDQSGSQEKL LVDSQGLSGC SPRDSGCYES SENLENGKTR KASLLSAKSS TEPSLKSFSR NQLGNYPTLP LMKSGDALKQ GQEEGRLGGG LAPDTSKSCD PPGVTGLNKN RRSLPVSICR SCETLEGPQT VDTWPRSHSL DDLQVEPGAE QDVPTEVTEP PPQIVPEVPQ KTTASSTKAQ PLEQDSAVDN ALLLTQSKRF SEPQKLTTKK LEGSIAASGR GLSPPQCLPR NYDAQPPGAK HGLARTPLEG HRKGHEFEGT HHPLGTKEGV DAEQRMQPKI PSQPPPVPAK KSRERLANGL HPVPMGPSGA LPSPDAPCLP VKRGSPASPT SPSDCPPALA PRPLSGQAPG SPPSTRPPPW LSELPENTSL QEHGVKLGPA LTRKVSCARG VDLETLTENK LHAEGIDLTE EPYSDKHGRC GIPEALVQRY AEDLDQPERD VAANMDQIRV KQLRKQHRMA IPSGGLTEIC RKPVSPGCIS SVSDWLISIG LPMYAGTLST AGFSTLSQVP SLSHTCLQEA GITEERHIRK LLSAARLFKL PPGPEAM
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Attributs du produit: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

niveau d'endotoxine: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Commentaires:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: SASH1 (SAM and SH3 Domain Containing 1 (SASH1))

Autre désignation: SASH1 (SASH1 Produits)

Synonymes: fi33a01 Protein, MGC162728 Protein, tdsubc_2e1 Protein, wu:fi33a01 Protein, ik:tdsubc_2e1 Protein, xx:tdsubc_2e1 Protein, SH3D6A Protein, dJ323M4 Protein, dJ323M4.1 Protein, 1100001C18Rik Protein, 2500002E12Rik Protein, A330076K04Rik Protein, mKIAA0790 Protein, RGD1566017 Protein, SAM and SH3 domain containing 1a Protein, SAM and SH3 domain containing 1 Protein, sash1a Protein, SASH1 Protein, sash1 Protein, Sash1 Protein

Sujet: SAM and SH3 domain-containing protein 1 (Proline-glutamate repeat-containing protein),FUNCTION: Is a positive regulator of NF-kappa-B signaling downstream of TLR4 activation. It acts as a scaffold molecule to assemble a molecular complex that includes TRAF6, MAP3K7, CHUK and IKBKB, thereby facilitating NF-kappa-B signaling activation (PubMed:23776175). Regulates TRAF6 and MAP3K7 ubiquitination (PubMed:23776175). Involved in the regulation of cell mobility (PubMed:23333244, PubMed:23776175, PubMed:25315659). Regulates lipolysaccharide (LPS)-induced endothelial cell migration (PubMed:23776175). Is involved in the regulation of skin pigmentation through the control of melanocyte migration in the epidermis (PubMed:23333244). {ECO:0000269|PubMed:23333244, ECO:0000269|PubMed:23776175, ECO:0000269|PubMed:25315659}.

Poids moléculaire: 136.7 kDa

UniProt: O94885