SH3TC1 Protein (AA 1-1336) (His tag)
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- Antigène Tous les produits SH3TC1
- SH3TC1 (SH3 Domain and Tetratricopeptide Repeats 1 (SH3TC1))
- Type de proteíne
- Recombinant
- Attributs du protein
- AA 1-1336
- Origine
- Humain
- Source
- Cellules d'insectes
- Purification/Conjugué
- Cette SH3TC1 protéine est marqué à la His tag.
- Application
- Crystallization (Crys), ELISA, SDS-PAGE (SDS), Western Blotting (WB)
- Séquence
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MENLPAVTTE EPTPMGRGPV GPSGGGSTRD QVRTVVMRPS VSWEKAGPEE AKAPVRGDEA PPARVAGPAA GTPPCQMGVY PTDLTLQLLA VRRKSRLRDP GLQQTLRGQL RLLENDSREM ARVLGELSAR LLSIHSDQDR IVVTFKTFEE IWKFSTYHAL GFTHHCLANL LMDQAFWLLL PSEEEETAIQ VHVDENALRL THESLLIQEG PFFVLCPDHH VRVMTGPRDA GNGPQALRQA SGAPQGEAAP ETDSSPPSPS VSSEEVAVAA APEPLIPFHQ WALRIPQDPI DDAMGGPVMP GNPLMAVGLA SALADFQGSG PEEMTFRGGD LIEILGAQVP SLPWCVGRHA ASGRVGFVRS SLISMQGPVS ELESAIFLNE EEKSFFSEGC FSEEDARQLL RRMSGTDVCS VYSLDSVEEA ETEQPQEKEI PPPCLSLEPQ ETLQKVKNVL EQCKTCPGCP QEPASWGLCA ASSDVSLQDP EEPSFCLEAE DDWEDPEALS SLLLFLNAPG YKASFRGLYD VALPWLSSVF RSFSDEEELT GRLAQARGAA KKAGLLMALA RLCFLLGRLC SRRLKLSQAR VYFEEALGAL EGSFGDLFLV VAVYANLASI YRKQKNREKC AQVVPKAMAL LLGTPDHICS TEAEGELLQL ALRRAVGGQS LQAEARACFL LARHHVHLKQ PEEALPFLER LLLLHRDSGA PEAAWLSDCY LLLADIYSRK CLPHLVLSCV KVASLRTRGS LAGSLRSVNL VLQNAPQPHS LPAQTSHYLR QALASLTPGT GQALRGPLYT SLAQLYSHHG CHGPAITFMT QAVEASAIAG VRAIVDHLVA LAWLHVLHGQ SPVALDILQS VRDAVVASED QEGVIANMVA VALKRTGRTR QAAESYYRAL RVARDLGQQR NQAVGLANFG ALCLHAGASR LAQHYLLEAV RLFSRLPLGE CGRDFTHVLL QLGHLCTRQG PAQQGKGYYE WALLVAVEMG HVESQLRAVQ RLCHFYSAVM PSEAQCVIYH ELQLSLACKV ADKVLEGQLL ETISQLYLSL GTERAYKSAL DYTKRSLGIF IDLQKKEKEA HAWLQAGKIY YILRQSELVD LYIQVAQNVA LYTGDPNLGL ELFEAAGDIF FDGAWEREKA VSFYRDRALP LAVTTGNRKA ELRLCNKLVA LLATLEEPQE GLEFAHMALA LSITLGDRLN ERVAYHRLAA LQHRLGHGEL AEHFYLKALS LCNSPLEFDE ETLYYVKVYL VLGDIIFYDL KDPFDAAGYY QLALAAAVDL GNKKAQLKIY TRLATIYHNF LLDREKSLFF YQKARTFATE LNVRRVNLPP LPLCGWAPWL APSHPR
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us. - Attributs du produit
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- Made in Germany - from design to production - by highly experienced protein experts.
- Human SH3TC1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).
When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- Purification
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Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
- In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- Pureté
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- Stérilité
- 0.22 μm filtered
- niveau d'endotoxine
- Protein is endotoxin free.
- Classe de qualité
- Crystallography grade
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- Indications d'application
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
- Commentaires
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In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
- Conseil sur la manipulation
- Avoid repeated freeze-thaw cycles.
- Stock
- -80 °C
- Stockage commentaire
- Store at -80°C.
- Date de péremption
- Unlimited (if stored properly)
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- Antigène
- SH3TC1 (SH3 Domain and Tetratricopeptide Repeats 1 (SH3TC1))
- Autre désignation
- SH3TC1 (SH3TC1 Produits)
- Synonymes
- SH3 domain and tetratricopeptide repeats 1 Protein, SH3TC1 Protein
- Poids moléculaire
- 147.9 kDa Including tag.
- UniProt
- Q8TE82
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