SSRP1 Protein (AA 2-709) (His tag)

N° du produit ABIN3095607

Détail du produit

Antigène: SSRP1 (Structure Specific Recognition Protein 1 (SSRP1))

Type de proteíne: Recombinant

Attributs du protein: AA 2-709

Origine: Humain

Source: Cellules d'insectes

Purification/Conjugué: Cette SSRP1 protéine est marqué à la His tag.

Application: Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)

Séquence: AETLEFNDVY QEVKGSMNDG RLRLSRQGII FKNSKTGKVD NIQAGELTEG IWRRVALGHG LKLLTKNGHV YKYDGFRESE FEKLSDFFKT HYRLELMEKD LCVKGWNWGT VKFGGQLLSF DIGDQPVFEI PLSNVSQCTT GKNEVTLEFH QNDDAEVSLM EVRFYVPPTQ EDGVDPVEAF AQNVLSKADV IQATGDAICI FRELQCLTPR GRYDIRIYPT FLHLHGKTFD YKIPYTTVLR LFLLPHKDQR QMFFVISLDP PIKQGQTRYH FLILLFSKDE DISLTLNMNE EEVEKRFEGR LTKNMSGSLY EMVSRVMKAL VNRKITVPGN FQGHSGAQCI TCSYKASSGL LYPLERGFIY VHKPPVHIRF DEISFVNFAR GTTTTRSFDF EIETKQGTQY TFSSIEREEY GKLFDFVNAK KLNIKNRGLK EGMNPSYDEY ADSDEDQHDA YLERMKEEGK IREENANDSS DDSGEETDES FNPGEEEEDV AEEFDSNASA SSSSNEGDSD RDEKKRKQLK KAKMAKDRKS RKKPVEVKKG KDPNAPKRPM SAYMLWLNAS REKIKSDHPG ISITDLSKKA GEIWKGMSKE KKEEWDRKAE DARRDYEKAM KEYEGGRGES SKRDKSKKKK KVKVKMEKKS TPSRGSSSKS SSRQLSESFK SKEFVSSDES SSGENKSKKK RRRSEDSEEE ELASTPPSSE DSASGSDE
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Attributs du produit:

• Made in Germany - from design to production - by highly experienced protein experts.
• Human SSRP1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Protein is endotoxin free.

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Commentaires:

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: SSRP1 (Structure Specific Recognition Protein 1 (SSRP1))

Autre désignation: SSRP1 (SSRP1 Produits)

Sujet: Component of the FACT complex, a general chromatin factor that acts to reorganize nucleosomes. The FACT complex is involved in multiple processes that require DNA as a template such as mRNA elongation, DNA replication and DNA repair. During transcription elongation the FACT complex acts as a histone chaperone that both destabilizes and restores nucleosomal structure. It facilitates the passage of RNA polymerase II and transcription by promoting the dissociation of one histone H2A-H2B dimer from the nucleosome, then subsequently promotes the reestablishment of the nucleosome following the passage of RNA polymerase II. The FACT complex is probably also involved in phosphorylation of 'Ser-392' of p53/TP53 via its association with CK2 (casein kinase II). Binds specifically to double-stranded DNA and at low levels to DNA modified by the antitumor agent cisplatin. May potentiate cisplatin-induced cell death by blocking replication and repair of modified DNA. Also acts as a transcriptional coactivator for p63/TP63. {ECO:0000269|PubMed:10912001, ECO:0000269|PubMed:11239457, ECO:0000269|PubMed:12374749, ECO:0000269|PubMed:12934006, ECO:0000269|PubMed:16713563, ECO:0000269|PubMed:9489704, ECO:0000269|PubMed:9566881, ECO:0000269|PubMed:9836642}.

Poids moléculaire: 81.9 kDa Including tag.

UniProt: Q08945

Pathways: Chromatin Binding