TAF3 Protein (AA 1-929) (Strep Tag)

N° du produit ABIN3095797

Détail du produit

Antigène: TAF3 (TAF3 RNA Polymerase II, TATA Box Binding Protein (TBP)-Associated Factor, 140kDa (TAF3))

Type de proteíne: Recombinant

Attributs du protein: AA 1-929

Origine: Humain

Source: Tobacco (Nicotiana tabacum)

Purification/Conjugué: Cette TAF3 protéine est marqué à la Strep Tag.

Application: ELISA, SDS-PAGE (SDS), Western Blotting (WB)

Séquence: MCESYSRSLL RVSVAQICQA LGWDSVQLSA CHLLTDVLQR YLQQLGRGCH RYSELYGRTD PILDDVGEAF QLMGVSLHEL EDYIHNIEPV TFPHQIPSFP VSKNNVLQFP QPGSKDAEER KEYIPDYLPP IVSSQEEEEE EQVPTDGGTS AEAMQVPLEE DDELEEEEII NDENFLGKRP LDSPEAEELP AMKRPRLLST KGDTLDVVLL EAREPLSSIN TQKIPPMLSP VHVQDSTDLA PPSPEPPMLA PVAKSQMPTA KPLETKSFTP KTKTKTSSPG QKTKSPKTAQ SPAMVGSPIR SPKTVSKEKK SPGRSKSPKS PKSPKVTTHI PQTPVRPETP NRTPSATLSE KISKETIQVK QIQTPPDAGK LNSENQPKKA VVADKTIEAS IDAVIARACA EREPDPFEFS SGSESEGDIF TSPKRISGPE CTTPKASTSA NNFTKSGSTP LPLSGGTSSS DNSWTMDASI DEVVRKAKLG TPSNMPPNFP YISSPSVSPP TPEPLHKVYE EKTKLPSSVE VKKKLKKELK TKMKKKEKQR DREREKDKNK DKSKEKDKVK EKEKDKETGR ETKYPWKEFL KEEEADPYKF KIKEFEDVDP KVKLKDGLVR KEKEKHKDKK KDREKGKKDK DKREKEKVKD KGREDKMKAP APPLVLPPKE LALPLFSPAT ASRVPAMLPS LLPVLPEKLF EEKEKVKEKE KKKDKKEKKK KKEKEKEKKE KEREKEKRER EKREKEKEKH KHEKIKVEPV ALAPSPVIPR LTLRVGAGQD KIVISKVVPA PEAKPAPSQN RPKTPPPAPA PAPGPMLVSP APVPLPLLAQ AAAGPALLPS PGPAASGASA KAPVRSVVTE TVSTYVIRDE WGNQIWICPG CNKPDDGSPM IGCDDCDDWY HWPCVGIMTA PPEEMQWFCP KCANKKKDKK HKKRKHRAH
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Attributs du produit: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

niveau d'endotoxine: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Commentaires:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: TAF3 (TAF3 RNA Polymerase II, TATA Box Binding Protein (TBP)-Associated Factor, 140kDa (TAF3))

Autre désignation: TAF3 (TAF3 Produits)

Synonymes: BIP2 Protein, Bip2 Protein, CG2009 Protein, DmTAF3 Protein, Dmel\\CG2009 Protein, TAF155 Protein, TAF3 Protein, TAFII155 Protein, TAF[[II]]155 Protein, TFIID Protein, bip-II Protein, dBIP2 Protein, dTAFII3 Protein, dTAF[[II]]155 Protein, dmTAF3 Protein, i163 Protein, i31 Protein, fi76b06 Protein, wu:fi76b06 Protein, zgc:136280 Protein, 140kDa Protein, 4933439M23Rik Protein, AW539625 Protein, TAF140 Protein, TAFII-140 Protein, TAFII140 Protein, mTAFII140 Protein, CG2009 gene product from transcript CG2009-RA Protein, TAF3 RNA polymerase II, TATA box binding protein (TBP)-associated facto Protein, TATA-box binding protein associated factor 3 Protein, TATA-box binding protein associated factor 3 L homeolog Protein, bip2 Protein, taf3 Protein, Taf3 Protein, TAF3 Protein, taf3.L Protein

Sujet: Transcription initiation factor TFIID subunit 3 (140 kDa TATA box-binding protein-associated factor) (TBP-associated factor 3) (Transcription initiation factor TFIID 140 kDa subunit) (TAF(II)140) (TAF140) (TAFII-140) (TAFII140),FUNCTION: The TFIID basal transcription factor complex plays a major role in the initiation of RNA polymerase II (Pol II)-dependent transcription (PubMed:33795473). TFIID recognizes and binds promoters with or without a TATA box via its subunit TBP, a TATA-box-binding protein, and promotes assembly of the pre-initiation complex (PIC) (PubMed:33795473). The TFIID complex consists of TBP and TBP-associated factors (TAFs), including TAF1, TAF2, TAF3, TAF4, TAF5, TAF6, TAF7, TAF8, TAF9, TAF10, TAF11, TAF12 and TAF13 (PubMed:33795473). The TFIID complex structure can be divided into 3 modules TFIID-A, TFIID-B, and TFIID-C (PubMed:33795473). TAF3 forms the TFIID-A module together with TAF5 and TBP (PubMed:33795473). Required in complex with TBPL2 for the differentiation of myoblasts into myocytes (PubMed:11438666). The TAF3-TBPL2 complex replaces TFIID at specific promoters at an early stage in the differentiation process (PubMed:11438666). {ECO:0000269|PubMed:11438666, ECO:0000269|PubMed:33795473}.

Poids moléculaire: 103.6 kDa

UniProt: Q5VWG9

Pathways: Proton Transport, Ribonucleoside Biosynthetic Process, Maintenance of Protein Location