Transcription Factor 7-Like 2 (T-Cell Specific, HMG-Box) (TCF7L2) (AA 1-619) protein (His tag)

Détails pour le produit réf. ABIN3095869
Nom de la protéine
  • TCF4B
  • TCF4E
  • Tcf-4
  • Tcf4
  • mTcf-4B
  • mTcf-4E
  • tcf4
  • XTCF-4
  • tcf4e
  • TCF4
  • TCF-4
  • TCFL2
  • transcription factor 7 like 2
  • transcription factor 7-like 2
  • transcription factor 7 like 2, T cell specific, HMG box
  • transcription factor 7 like 2 L homeolog
  • transcription factor 7-like 2 (T-cell specific, HMG-box)
  • TCF7L2
  • LOC100545943
  • Tcf7l2
  • tcf7l2
  • tcf7l2.L
Attributs du protein
AA 1-619
1
1
1
Origine
Humain
2
1
Source
Escherichia coli (E. coli)
2
1
Type de proteíne
Recombinant
Purification/Conjugué
Cette TCF7L2 protéine est marqué à la His tag.
Application
Crystallization (Crys), ELISA, SDS-PAGE (SDS), Western Blotting (WB)
Options
Séquence MPQLNGGGGD DLGANDELIS FKDEGEQEEK SSENSSAERD LADVKSSLVN ESETNQNSSS DSEAERRPPP RSESFRDKSR ESLEEAAKRQ DGGLFKGPPY PGYPFIMIPD LTSPYLPNGS LSPTARTLHF QSGSTHYSAY KTIEHQIAVQ YLQMKWPLLD VQAGSLQSRQ ALKDARSPSP AHIVSNKVPV VQHPHHVHPL TPLITYSNEH FTPGNPPPHL PADVDPKTGI PRPPHPPDIS PYYPLSPGTV GQIPHPLGWL VPQQGQPVYP ITTGGFRHPY PTALTVNASM SRFPPHMVPP HHTLHTTGIP HPAIVTPTVK QESSQSDVGS LHSSKHQDSK KEEEKKKPHI KKPLNAFMLY MKEMRAKVVA ECTLKESAAI NQILGRRWHA LSREEQAKYY ELARKERQLH MQLYPGWSAR DNYGKKKKRK RDKQPGETNE HSECFLNPCL SLPPITDLSA PKKCRARFGL DQQNNWCGPC RRKKKCVRYI QGEGSCLSPP SSDGSLLDSP PPSPNLLGSP PRDAKSQTEQ TQPLSLSLKP DPLAHLSMMP PPPALLLAEA THKASALCPN GALDLPPAAL QPAAPSSSIA QPSTSSLHSH SSLAGTQPQP LSLVTKSLEH HHHHH
Sequence including C-terminal His-tag.
Attributs du produit
  • Made in Germany - from design to production - by highly experienced protein experts.
  • Human TCF7L2 Protein (raised in E. Coli) purified by multi-step, protein-specific process to ensure crystallization grade.
  • State-of-the-art algorithm used for plasmid design (Gene synthesis).
  • Tag Location: C-terminal His Tag
Purification The protein is purified from the cleared cell lysate using EDTA resistant His-tag capture materials. Eluate fractions are analyzed by SDS-PAGE. Protein containing fractions are subjected to a second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
Pureté >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
Stérilité 0.22 μm filtered
niveau d'endotoxine Endotoxin has not been removed. Please contact us if you require endotoxin removal.
Classe de qualité Crystallography grade
Nom de la protéine
Sujet Participates in the Wnt signaling pathway and modulates MYC expression by binding to its promoter in a sequence-specific manner. Acts as repressor in the absence of CTNNB1, and as activator in its presence. Activates transcription from promoters with several copies of the Tcf motif 5'-CCTTTGATC-3' in the presence of CTNNB1. TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by TCF7L2/TCF4 and CTNNB1. Expression of dominant-negative mutants results in cell-cycle arrest in G1. Necessary for the maintenance of the epithelial stem-cell compartment of the small intestine. {ECO:0000269|PubMed:12408868, ECO:0000269|PubMed:12727872, ECO:0000269|PubMed:19443654, ECO:0000269|PubMed:22699938, ECO:0000269|PubMed:9727977}.
Poids moléculaire 68.9 kDa Including tag.
UniProt Q9NQB0
Pathways Signalisation WNT, Positive Regulation of Peptide Hormone Secretion, Peptide Hormone Metabolism, Regulation of Hormone Metabolic Process, Carbohydrate Homeostasis, Stem Cell Maintenance, Protein targeting to Nucleus
Indications d'application In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
Commentaires

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions For Research Use only
Format Liquid
Buffer 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
Conseil sur la manipulation Avoid repeated freeze-thaw cycles.
Stock -80 °C
Stockage commentaire Store at -80°C.
Date de péremption Unlimited (if stored properly)
Images (Fournisseur)
 image for Transcription Factor 7-Like 2 (T-Cell Specific, HMG-Box) (TCF7L2) (AA 1-619) protein (His tag) (ABIN3095869) „Crystallography Grade“ protein due to multi-step, protein-specific purification process
Size-exclusion chromatography-High Pressure Liquid Chromatography (SEC-HPLC) image for Transcription Factor 7-Like 2 (T-Cell Specific, HMG-Box) (TCF7L2) (AA 1-619) protein (His tag) (ABIN3095869) Transcription Factor 7-Like 2 (T-Cell Specific, HMG-Box) (TCF7L2) (AA 1-619) protein (His tag) (Image 2)
Western Blotting (WB) image for Transcription Factor 7-Like 2 (T-Cell Specific, HMG-Box) (TCF7L2) (AA 1-619) protein (His tag) (ABIN3095869) Transcription Factor 7-Like 2 (T-Cell Specific, HMG-Box) (TCF7L2) (AA 1-619) protein (His tag) (Image 3)
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