U2AF2 Protein (AA 2-475) (His tag)

N° du produit ABIN3096118

Détail du produit

Antigène: U2AF2 (U2AF59) (U2 Small Nuclear RNA Auxiliary Factor 2 (U2AF59))

Type de proteíne: Recombinant

Attributs du protein: AA 2-475

Origine: Humain

Source: Cellules d'insectes

Purification/Conjugué: Cette U2AF2 protéine est marqué à la His tag.

Application: ELISA, Western Blotting (WB), Crystallization (Crys), SDS-PAGE (SDS)

Séquence: SDFDEFERQL NENKQERDKE NRHRKRSHSR SRSRDRKRRS RSRDRRNRDQ RSASRDRRRR SKPLTRGAKE EHGGLIRSPR HEKKKKVRKY WDVPPPGFEH ITPMQYKAMQ AAGQIPATAL LPTMTPDGLA VTPTPVPVVG SQMTRQARRL YVGNIPFGIT EEAMMDFFNA QMRLGGLTQA PGNPVLAVQI NQDKNFAFLE FRSVDETTQA MAFDGIIFQG QSLKIRRPHD YQPLPGMSEN PSVYVPGVVS TVVPDSAHKL FIGGLPNYLN DDQVKELLTS FGPLKAFNLV KDSATGLSKG YAFCEYVDIN VTDQAIAGLN GMQLGDKKLL VQRASVGAKN ATLVSPPSTI NQTPVTLQVP GLMSSQVQMG GHPTEVLCLM NMVLPEELLD DEEYEEIVED VRDECSKYGL VKSIEIPRPV DGVEVPGCGK IFVEFTSVFD CQKAMQGLTG RKFANRVVVT KYCDPDSYHR RDFW
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Attributs du produit:

• Made in Germany - from design to production - by highly experienced protein experts.
• Human U2AF2 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Protein is endotoxin free.

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Commentaires:

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: U2AF2 (U2AF59) (U2 Small Nuclear RNA Auxiliary Factor 2 (U2AF59))

Autre désignation: U2AF2 (U2AF59 Produits)

Synonymes: U2AF65 Protein, 65kDa Protein, u2af65 Protein, U2AF2 Protein, DDBDRAFT_0186947 Protein, DDBDRAFT_0233353 Protein, DDB_0186947 Protein, DDB_0233353 Protein, zgc:110115 Protein, U2 small nuclear RNA auxiliary factor 2 Protein, U2 small nuclear ribonucleoprotein auxiliary factor (U2AF) 2 Protein, U2 small nuclear RNA auxiliary factor 2 S homeolog Protein, RNA recognition motif-containing protein RRM Protein, Splicing factor U2AF 65 kDa subunit Protein, U2 small nuclear RNA auxiliary factor 2a Protein, U2AF2 Protein, U2af2 Protein, u2af2.S Protein, u2af2 Protein, u2af2a Protein

Sujet: Necessary for the splicing of pre-mRNA. By recruiting PRPF19 and the PRP19C/Prp19 complex/NTC/Nineteen complex to the RNA polymerase II C-terminal domain (CTD), and thereby pre-mRNA, may couple transcription to splicing (PubMed:21536736). Induces cardiac troponin-T (TNNT2) pre-mRNA exon inclusion in muscle. Regulates the TNNT2 exon 5 inclusion through competition with MBNL1. Binds preferentially to a single-stranded structure within the polypyrimidine tract of TNNT2 intron 4 during spliceosome assembly. Required for the export of mRNA out of the nucleus, even if the mRNA is encoded by an intron-less gene. Represses the splicing of MAPT/Tau exon 10. {ECO:0000269|PubMed:15009664, ECO:0000269|PubMed:19470458, ECO:0000269|PubMed:19574390, ECO:0000269|PubMed:21536736}.

Poids moléculaire: 54.3 kDa Including tag.

UniProt: P26368