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UFM1-Specific Ligase 1 (UFL1) (AA 2-794) protein (His tag) Protein

UFL1 Origine: Humain Source: Cellules d'insectes Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. Crys, ELISA, SDS, WB
N° du produit ABIN3096158
$13,376.85
Plus frais de port $45.00 et $22.00 pour carboglace
1 mg ABIN3096158
1 mg ABIN3096158
local_shipping Destination: Etats-Unis
Envoi sous 70 jours ouvrables
  • Nom de la protéine
    Type de proteíne
    Recombinant
    Attributs du protein
    AA 2-794
    Origine
    Humain
    Source
    Cellules d'insectes
    Purification/Conjugué
    Cette UFL1 protéine est marqué à la His tag.
    Application
    Crystallization (Crys), ELISA, SDS-PAGE (SDS), Western Blotting (WB)
    Séquence
    ADAWEEIRRL AADFQRAQFA EATQRLSERN CIEIVNKLIA QKQLEVVHTL DGKEYITPAQ ISKEMRDELH VRGGRVNIVD LQQVINVDLI HIENRIGDII KSEKHVQLVL GQLIDENYLD RLAEEVNDKL QESGQVTISE LCKTYDLPGN FLTQALTQRL GRIISGHIDL DNRGVIFTEA FVARHKARIR GLFSAITRPT AVNSLISKYG FQEQLLYSVL EELVNSGRLR GTVVGGRQDK AVFVPDIYSR TQSTWVDSFF RQNGYLEFDA LSRLGIPDAV SYIKKRYKTT QLLFLKAACV GQGLVDQVEA SVEEAISSGT WVDIAPLLPT SLSVEDAAIL LQQVMRAFSK QASTVVFSDT VVVSEKFIND CTELFRELMH QKAEKEMKNN PVHLITEEDL KQISTLESVS TSKKDKKDER RRKATEGSGS MRGGGGGNAR EYKIKKVKKK GRKDDDSDDE SQSSHTGKKK PEISFMFQDE IEDFLRKHIQ DAPEEFISEL AEYLIKPLNK TYLEVVRSVF MSSTTSASGT GRKRTIKDLQ EEVSNLYNNI RLFEKGMKFF ADDTQAALTK HLLKSVCTDI TNLIFNFLAS DLMMAVDDPA AITSEIRKKI LSKLSEETKV ALTKLHNSLN EKSIEDFISC LDSAAEACDI MVKRGDKKRE RQILFQHRQA LAEQLKVTED PALILHLTSV LLFQFSTHSM LHAPGRCVPQ IIAFLNSKIP EDQHALLVKY QGLVVKQLVS QSKKTGQGDY PLNNELDKEQ EDVASTTRKE LQELSSSIKD LVLKSRKSSV TEE
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Attributs du produit
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Human UFL1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Purification
    Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Pureté
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Stérilité
    0.22 μm filtered
    niveau d'endotoxine
    Protein is endotoxin free.
    Classe de qualité
    Crystallography grade
  • Indications d'application
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    Commentaires

    In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Conseil sur la manipulation
    Avoid repeated freeze-thaw cycles.
    Stock
    -80 °C
    Stockage commentaire
    Store at -80°C.
    Date de péremption
    Unlimited (if stored properly)
  • Nom de la protéine
    Sujet
    E3 protein ligase that mediates ufmylation, the covalent attachment of the ubiquitin-like modifier UFM1 to substrate proteins, a post-translational modification on lysine residues of proteins that may play a crucial role in a number of cellular processes. Mediates DDRGK1 ufmylation and may regulate the proteasomal degradation of DDRGK1 and CDK5RAP3 thereby modulating NF-kappa-B signaling (PubMed:20018847, PubMed:20164180, PubMed:20228063, PubMed:25219498). May also through TRIP4 ufmylation play a role in nuclear receptors-mediated transcription (PubMed:25219498). May play a role in the unfolded protein response, mediating the ufmylation of multiple proteins in response to endoplasmic reticulum stress (PubMed:23152784). {ECO:0000269|PubMed:20018847, ECO:0000269|PubMed:20164180, ECO:0000269|PubMed:20228063, ECO:0000269|PubMed:23152784, ECO:0000269|PubMed:25219498}.
    Poids moléculaire
    90.4 kDa Including tag.
    UniProt
    O94874
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