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VPS11 Protein (AA 2-941) (His tag)

Crystallography grade VPS11 Origine: Humain Hôte: Cellules d'insectes Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, Crys, SDS
N° du produit ABIN3096310
  • Antigène Voir toutes VPS11 Protéines
    VPS11 (Vacuolar Protein Sorting 11 Homolog (VPS11))
    Type de proteíne
    Recombinant
    Attributs du protein
    AA 2-941
    Origine
    Humain
    Source
    • 2
    • 1
    Cellules d'insectes
    Purification/Conjugué
    Cette VPS11 protéine est marqué à la His tag.
    Application
    ELISA, Western Blotting (WB), Crystallization (Crys), SDS-PAGE (SDS)
    Séquence
    AAYLQWRRFV FFDKELVKEP LSNDGAAPGA TPASGSAASK FLCLPPGITV CDSGRGSLVF GDMEGQIWFL PRSLQLTGFQ AYKLRVTHLY QLKQHNILAS VGEDEEGINP LVKIWNLEKR DGGNPLCTRI FPAIPGTEPT VVSCLTVHEN LNFMAIGFTD GSVTLNKGDI TRDRHSKTQI LHKGNYPVTG LAFRQAGKTT HLFVVTTENV QSYIVSGKDY PRVELDTHGC GLRCSALSDP SQDLQFIVAG DECVYLYQPD ERGPCFAFEG HKLIAHWFRG YLIIVSRDRK VSPKSEFTSR DSQSSDKQIL NIYDLCNKFI AYSTVFEDVV DVLAEWGSLY VLTRDGRVHA LQEKDTQTKL EMLFKKNLFE MAINLAKSQH LDSDGLAQIF MQYGDHLYSK GNHDGAVQQY IRTIGKLEPS YVIRKFLDAQ RIHNLTAYLQ TLHRQSLANA DHTTLLLNCY TKLKDSSKLE EFIKKKSESE VHFDVETAIK VLRQAGYYSH ALYLAENHAH HEWYLKIQLE DIKNYQEALR YIGKLPFEQA ESNMKRYGKI LMHHIPEQTT QLLKGLCTDY RPSLEGRSDR EAPGCRANSE EFIPIFANNP RELKAFLEHM SEVQPDSPQG IYDTLLELRL QNWAHEKDPQ VKEKLHAEAI SLLKSGRFCD VFDKALVLCQ MHDFQDGVLY LYEQGKLFQQ IMHYHMQHEQ YRQVISVCER HGEQDPSLWE QALSYFARKE EDCKEYVAAV LKHIENKNLM PPLLVVQTLA HNSTATLSVI RDYLVQKLQK QSQQIAQDEL RVRRYREETT RIRQEIQELK ASPKIFQKTK CSICNSALEL PSVHFLCGHS FHQHCFESYS ESDADCPTCL PENRKVMDMI RAQEQKRDLH DQFQHQLRCS NDSFSVIADY FGRGVFNKLT LLTDPPTARL TSSLEAGLQR DLLMHSRRGT
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Attributs du produit
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Human VPS11 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Purification
    Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Pureté
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Stérilité
    0.22 μm filtered
    niveau d'endotoxine
    Protein is endotoxin free.
    Classe de qualité
    Crystallography grade
    Top Product
    Discover our top product VPS11 Protéine
  • Indications d'application
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    Commentaires

    In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Conseil sur la manipulation
    Avoid repeated freeze-thaw cycles.
    Stock
    -80 °C
    Stockage commentaire
    Store at -80°C.
    Date de péremption
    Unlimited (if stored properly)
  • Antigène
    VPS11 (Vacuolar Protein Sorting 11 Homolog (VPS11))
    Autre désignation
    VPS11 (VPS11 Produits)
    Synonymes
    zgc:112329 Protein, ATVPS11 Protein, F5G3.7 Protein, F5G3_7 Protein, vacuolar protein sorting 11 Protein, DDBDRAFT_0204454 Protein, DDBDRAFT_0234135 Protein, DDB_0204454 Protein, DDB_0234135 Protein, end1 Protein, pep5 Protein, hvps11 Protein, rnf108 Protein, 1200011A11Rik Protein, END1 Protein, PEP5 Protein, RNF108 Protein, hVPS11 Protein, VPS11, CORVET/HOPS core subunit Protein, vacuolar protein sorting 11 Protein, vacuolar protein sorting protein 11 Protein, VPS11, CORVET/HOPS core subunit S homeolog Protein, Vacuolar peripheral membrane protein similar to S. cerevisiae PEP5 (YMR231W); C terminus is almost identical to nonallelic CaP19.1278 Protein, 7-fold repeat in clathrin and VPS proteins repeat-containing protein Protein, vacuolar protein sorting-associated protein 11 homolog Protein, VPS11 CORVET/HOPS core subunit Protein, VPS11 Protein, vps11 Protein, MCYG_02909 Protein, vps11.S Protein, LOC100632536 Protein, Vps11 Protein
    Sujet
    Plays a role in vesicle-mediated protein trafficking to lysosomal compartments including the endocytic membrane transport and autophagic pathways. Believed to act as a core component of the putative HOPS and CORVET endosomal tethering complexes which are proposed to be involved in the Rab5-to-Rab7 endosome conversion probably implicating MON1A/B, and via binding SNAREs and SNARE complexes to mediate tethering and docking events during SNARE-mediated membrane fusion. The HOPS complex is proposed to be recruited to Rab7 on the late endosomal membrane and to regulate late endocytic, phagocytic and autophagic traffic towards lysosomes. The CORVET complex is proposed to function as a Rab5 effector to mediate early endosome fusion probably in specific endosome subpopulations (PubMed:11382755, PubMed:23351085, PubMed:24554770, PubMed:25266290, PubMed:25783203). Required for fusion of endosomes and autophagosomes with lysosomes (PubMed:25783203). Involved in cargo transport from early to late endosomes and required for the transition from early to late endosomes (PubMed:21148287). Involved in the retrograde Shiga toxin transport (PubMed:23593995). {ECO:0000269|PubMed:21148287, ECO:0000269|PubMed:23593995, ECO:0000269|PubMed:25783203, ECO:0000305|PubMed:11382755, ECO:0000305|PubMed:23351085, ECO:0000305|PubMed:24554770, ECO:0000305|PubMed:25266290, ECO:0000305|PubMed:25783203}.
    Poids moléculaire
    108.7 kDa Including tag.
    UniProt
    Q9H270
    Pathways
    SARS-CoV-2 Protein Interactome
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