VPS18 Protein (AA 2-973) (His tag)

N° du produit ABIN3096367

Détail du produit

Antigène: VPS18 (Vacuolar Protein Sorting 18 Homolog (VPS18))

Type de proteíne: Recombinant

Attributs du protein: AA 2-973

Origine: Humain

Source: Cellules d'insectes

Purification/Conjugué: Cette VPS18 protéine est marqué à la His tag.

Application: ELISA, Western Blotting (WB), Crystallization (Crys), SDS-PAGE (SDS)

Séquence: ASILDEYENS LSRSAVLQPG CPSVGIPHSG YVNAQLEKEV PIFTKQRIDF TPSERITSLV VSSNQLCMSL GKDTLLRIDL GKANEPNHVE LGRKDDAKVH KMFLDHTGSH LLIALSSTEV LYVNRNGQKV RPLARWKGQL VESVGWNKAL GTESSTGPIL VGTAQGHIFE AELSASEGGL FGPAPDLYFR PLYVLNEEGG PAPVCSLEAE RGPDGRSFVI ATTRQRLFQF IGRAAEGAEA QGFSGLFAAY TDHPPPFREF PSNLGYSELA FYTPKLRSAP RAFAWMMGDG VLYGALDCGR PDSLLSEERV WEYPEGVGPG ASPPLAIVLT QFHFLLLLAD RVEAVCTLTG QVVLRDHFLE KFGPLKHMVK DSSTGQLWAY TERAVFRYHV QREARDVWRT YLDMNRFDLA KEYCRERPDC LDTVLAREAD FCFRQRRYLE SARCYALTQS YFEEIALKFL EARQEEALAE FLQRKLASLK PAERTQATLL TTWLTELYLS RLGALQGDPE ALTLYRETKE CFRTFLSSPR HKEWLFASRA SIHELLASHG DTEHMVYFAV IMQDYERVVA YHCQHEAYEE ALAVLARHRD PQLFYKFSPI LIRHIPRQLV DAWIEMGSRL DARQLIPALV NYSQGGEVQQ VSQAIRYMEF CVNVLGETEQ AIHNYLLSLY ARGRPDSLLA YLEQAGASPH RVHYDLKYAL RLCAEHGHHR ACVHVYKVLE LYEEAVDLAL QVDVDLAKQC ADLPEEDEEL RKKLWLKIAR HVVQEEEDVQ TAMACLASCP LLKIEDVLPF FPDFVTIDHF KEAICSSLKA YNHHIQELQR EMEEATASAQ RIRRDLQELR GRYGTVEPQD KCATCDFPLL NRPFYLFLCG HMFHADCLLQ AVRPGLPAYK QARLEELQRK LGAAPPPAKG SARAKEAEGG AATAGPSREQ LKADLDELVA AECVYCGELM IRSIDRPFID PQRYEEEQLS WL
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Attributs du produit:

• Made in Germany - from design to production - by highly experienced protein experts.
• Human VPS18 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Protein is endotoxin free.

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Commentaires:

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: VPS18 (Vacuolar Protein Sorting 18 Homolog (VPS18))

Autre désignation: VPS18 (VPS18 Produits)

Synonymes: MGC82750 Protein, VPS18 Protein, DDBDRAFT_0190675 Protein, DDBDRAFT_0234134 Protein, DDB_0190675 Protein, DDB_0234134 Protein, 9930024E13Rik Protein, PEP3 Protein, chunp6929 Protein, fj35h10 Protein, hm:zehl0587 Protein, wu:fj35h10 Protein, VPS18, CORVET/HOPS core subunit Protein, vacuolar protein sorting protein 18 Protein, VPS18, CORVET/HOPS core subunit S homeolog Protein, subunit of the VPS-C complex Protein, 7-fold repeat in clathrin and VPS proteins repeat-containing protein Protein, vacuolar protein sorting 18 homolog (S. cerevisiae) Protein, VPS18 CORVET/HOPS core subunit Protein, VPS18 Protein, Tc00.1047053506401.70 Protein, vps18.S Protein, vps18 Protein, Vps18 Protein

Sujet: Plays a role in vesicle-mediated protein trafficking to lysosomal compartments including the endocytic membrane transport and autophagic pathways. Believed to act as a core component of the putative HOPS and CORVET endosomal tethering complexes which are proposed to be involved in the Rab5-to-Rab7 endosome conversion probably implicating MON1A/B, and via binding SNAREs and SNARE complexes to mediate tethering and docking events during SNARE-mediated membrane fusion. The HOPS complex is proposed to be recruited to Rab7 on the late endosomal membrane and to regulate late endocytic, phagocytic and autophagic traffic towards lysosomes. The CORVET complex is proposed to function as a Rab5 effector to mediate early endosome fusion probably in specific endosome subpopulations (PubMed:11382755, PubMed:23351085, PubMed:24554770, PubMed:25783203). Required for fusion of endosomes and autophagosomes with lysosomes (PubMed:25783203). Involved in dendrite development of Pukinje cells (By similarity). {ECO:0000250|UniProtKB:Q8R307, ECO:0000269|PubMed:25783203, ECO:0000305|PubMed:11382755, ECO:0000305|PubMed:23351085, ECO:0000305|PubMed:25783203}.

Poids moléculaire: 111.0 kDa Including tag.

UniProt: Q9P253

Pathways: Asymmetric Protein Localization