YES1 Protein (AA 2-543) (His tag)

N° du produit ABIN3096421

Détail du produit

Antigène: YES1 (V-Yes-1 Yamaguchi Sarcoma Viral Oncogene Homolog 1 (YES1))

Type de proteíne: Recombinant

Attributs du protein: AA 2-543

Origine: Humain

Source: Cellules d'insectes

Purification/Conjugué: Cette YES1 protéine est marqué à la His tag.

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS), Crystallization (Crys)

Séquence: GCIKSKENKS PAIKYRPENT PEPVSTSVSH YGAEPTTVSP CPSSSAKGTA VNFSSLSMTP FGGSSGVTPF GGASSSFSVV PSSYPAGLTG GVTIFVALYD YEARTTEDLS FKKGERFQII NNTEGDWWEA RSIATGKNGY IPSNYVAPAD SIQAEEWYFG KMGRKDAERL LLNPGNQRGI FLVRESETTK GAYSLSIRDW DEIRGDNVKH YKIRKLDNGG YYITTRAQFD TLQKLVKHYT EHADGLCHKL TTVCPTVKPQ TQGLAKDAWE IPRESLRLEV KLGQGCFGEV WMGTWNGTTK VAIKTLKPGT MMPEAFLQEA QIMKKLRHDK LVPLYAVVSE EPIYIVTEFM SKGSLLDFLK EGDGKYLKLP QLVDMAAQIA DGMAYIERMN YIHRDLRAAN ILVGENLVCK IADFGLARLI EDNEYTARQG AKFPIKWTAP EAALYGRFTI KSDVWSFGIL QTELVTKGRV PYPGMVNREV LEQVERGYRM PCPQGCPESL HELMNLCWKK DPDERPTFEY IQSFLEDYFT ATEPQYQPGE NL
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Attributs du produit:

• Made in Germany - from design to production - by highly experienced protein experts.
• Human YES1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Protein is endotoxin free.

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Commentaires:

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: YES1 (V-Yes-1 Yamaguchi Sarcoma Viral Oncogene Homolog 1 (YES1))

Autre désignation: YES1 (YES1 Produits)

Synonymes: HsT441 Protein, P61-YES Protein, Yes Protein, c-yes Protein, yes Protein, p61-Yes Protein, hst441 Protein, p61-yes Protein, YES Protein, c-Yes Protein, p60c-yes Protein, YES proto-oncogene 1, Src family tyrosine kinase Protein, YES proto-oncogene 1, Src family tyrosine kinase L homeolog Protein, YES1 Protein, yes1.L Protein, yes1 Protein, Yes1 Protein

Sujet: Non-receptor protein tyrosine kinase that is involved in the regulation of cell growth and survival, apoptosis, cell-cell adhesion, cytoskeleton remodeling, and differentiation. Stimulation by receptor tyrosine kinases (RTKs) including EGRF, PDGFR, CSF1R and FGFR leads to recruitment of YES1 to the phosphorylated receptor, and activation and phosphorylation of downstream substrates. Upon EGFR activation, promotes the phosphorylation of PARD3 to favor epithelial tight junction assembly. Participates in the phosphorylation of specific junctional components such as CTNND1 by stimulating the FYN and FER tyrosine kinases at cell-cell contacts. Upon T-cell stimulation by CXCL12, phosphorylates collapsin response mediator protein 2/DPYSL2 and induces T-cell migration. Participates in CD95L/FASLG signaling pathway and mediates AKT-mediated cell migration. Plays a role in cell cycle progression by phosphorylating the cyclin-dependent kinase 4/CDK4 thus regulating the G1 phase. Also involved in G2/M progression and cytokinesis. {ECO:0000269|PubMed:11901164, ECO:0000269|PubMed:18479465, ECO:0000269|PubMed:19276087, ECO:0000269|PubMed:21566460, ECO:0000269|PubMed:21713032}.

Poids moléculaire: 61.6 kDa Including tag.

UniProt: P07947

Pathways: CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Thromboxane A2 Receptor Signaling