TRIM59 Protein (AA 1-403) (Strep Tag)

N° du produit ABIN3102849

Détail du produit

Antigène: TRIM59 (Tripartite Motif Containing 59 (TRIM59))

Type de proteíne: Recombinant

Attributs du protein: AA 1-403

Origine: Humain

Source: Tobacco (Nicotiana tabacum)

Purification/Conjugué: Cette TRIM59 protéine est marqué à la Strep Tag.

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Séquence: MHNFEEELTC PICYSIFEDP RVLPCSHTFC RNCLENILQA SGNFYIWRPL RIPLKCPNCR SITEIAPTGI ESLPVNFALR AIIEKYQQED HPDIVTCPEH YRQPLNVYCL LDKKLVCGHC LTIGQHHGHP IDDLQSAYLK EKDTPQKLLE QLTDTHWTDL THLIEKLKEQ KSHSEKMIQG DKEAVLQYFK ELNDTLEQKK KSFLTALCDV GNLINQEYTP QIERMKEIRE QQLELMALTI SLQEESPLKF LEKVDDVRQH VQILKQRPLP EVQPVEIYPR VSKILKEEWS RTEIGQIKNV LIPKMKISPK RMSCSWPGKD EKEVEFLKIL NIVVVTLISV ILMSILFFNQ HIITFLSEIT LIWFSEASLS VYQSLSNSLH KVKNILCHIF YLLKEFVWKI VSH
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Attributs du produit: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

niveau d'endotoxine: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Commentaires:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: TRIM59 (Tripartite Motif Containing 59 (TRIM59))

Autre désignation: TRIM59 (TRIM59 Produits)

Synonymes: zgc:193694 Protein, zgc:193700 Protein, TRIM59 Protein, MRF1 Protein, RNF104 Protein, TRIM57 Protein, TSBF1 Protein, 2310035M22Rik Protein, 2700022F13Rik Protein, Mrf1 Protein, RGD1311956 Protein, LYR motif containing 9 Protein, tripartite motif containing 59 Protein, tripartite motif-containing 59 Protein, lyrm9 Protein, TRIM59 Protein, Trim59 Protein

Sujet: Tripartite motif-containing protein 59 (EC 2.3.2.27) (RING finger protein 104) (Tumor suppressor TSBF-1),FUNCTION: E3 ubiquitin ligase involved in different processes such as development and immune response (PubMed:22588174, PubMed:30231667). Serves as a negative regulator for innate immune signaling pathways by suppressing RLR-induced activation of IRF3/7 and NF-kappa-B via interaction with adapter ECSIT (PubMed:22588174). Regulates autophagy through modulating both the transcription and the ubiquitination of BECN1 (PubMed:30231667). On the one hand, regulates the transcription of BECN1 through negatively modulating the NF-kappa-B pathway. On the other hand, regulates TRAF6-mediated 'Lys-63'-linked ubiquitination of BECN1, thus affecting the formation of the BECN1-PIK3C3 complex. In addition, mediates 'Lys-48'-linked ubiquitination of TRAF6 and thereby promotes TRAF6 proteasomal degradation (PubMed:30231667). Acts also as a critical regulator for early embryo development from blastocyst stage to gastrula through modulating F-actin assembly and WASH1 'Lys-63'-linked ubiquitination (By similarity). {ECO:0000250|UniProtKB:Q922Y2, ECO:0000269|PubMed:22588174, ECO:0000269|PubMed:30231667}.

Poids moléculaire: 47.1 kDa

UniProt: Q8IWR1

Pathways: SARS-CoV-2 Protein Interactome