Tetraspanin 17 Protein (TSPAN17) (AA 1-270) (Strep Tag)

N° du produit ABIN3105092

Détail du produit

Antigène: Tetraspanin 17 (TSPAN17)

Type de proteíne: Recombinant

Attributs du protein: AA 1-270

Origine: Humain

Source: Tobacco (Nicotiana tabacum)

Purification/Conjugué: Cette Tetraspanin 17 protéine est marqué à la Strep Tag.

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Séquence: MPGKHQHFQE PEVGCCGKYF LFGFNIVFWV LGALFLAIGL WAWGEKGVLS NISALTDLGG LDPVWLFVVV GGVMSVLGFA GCIGALRENT FLLKFFSVFL GLIFFLELAT GILAFVFKDW IRDQLNLFIN NNVKAYRDDI DLQNLIDFAQ EYWSCCGARG PNDWNLNIYF NCTDLNPSRE RCGVPFSCCV RDPAEDVLNT QCGYDVRLKL ELEQQGFIHT KGCVGQFEKW LQDNLIVVAG VFMGIALLQI FGICLAQNLV SDIKAVKANW
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Attributs du produit: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

niveau d'endotoxine: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Commentaires:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: Tetraspanin 17 (TSPAN17)

Autre désignation: TSPAN17 (TSPAN17 Produits)

Synonymes: fc49c03 Protein, wu:fc49c03 Protein, zgc:158284 Protein, Tspan-17 Protein, fbxo23 Protein, MGC89422 Protein, GB11399 Protein, tetraspanin 17 Protein, FBX23 Protein, FBXO23 Protein, TM4SF17 Protein, 2210021G21Rik Protein, AI047581 Protein, Fbxo23 Protein, Tm4sf17 Protein, GHB-R Protein, tetraspanin 17 Protein, tetraspanin 17 S homeolog Protein, CD63 antigen Protein, tetraspanin-17 protein Protein, tspan17 Protein, tspan17.S Protein, TSPAN17 Protein, LOC552721 Protein, TET17 Protein, Tspan17 Protein

Sujet: Tetraspanin-17 (Tspan-17) (F-box only protein 23) (Tetraspan protein SB134) (Transmembrane 4 superfamily member 17),FUNCTION: Part of TspanC8 subgroup, composed of 6 members that interact with the transmembrane metalloprotease ADAM10. This interaction is required for ADAM10 exit from the endoplasmic reticulum and for enzymatic maturation and trafficking to the cell surface as well as substrate specificity. Different TspanC8/ADAM10 complexes have distinct substrates (PubMed:28600292, PubMed:37516108). Seems to regulate VE-cadherin expression in endothelial cells probably through interaction with ADAM10, promoting leukocyte transmigration (PubMed:28600292). {ECO:0000269|PubMed:28600292, ECO:0000269|PubMed:37516108}.

Poids moléculaire: 30.3 kDa

UniProt: Q96FV3