SLC46A2 Protein (AA 1-475) (Strep Tag)

N° du produit ABIN3106267

Détail du produit

Antigène: SLC46A2 (Solute Carrier Family 46 Member 2 (SLC46A2))

Type de proteíne: Recombinant

Attributs du protein: AA 1-475

Origine: Humain

Source: Tobacco (Nicotiana tabacum)

Purification/Conjugué: Cette SLC46A2 protéine est marqué à la Strep Tag.

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Séquence: MSPEVTCPRR GHLPRFHPRT WVEPVVASSQ VAASLYDAGL LLVVKASYGT GGSSNHSASP SPRGALEDQQ QRAISNFYII YNLVVGLSPL LSAYGLGWLS DRYHRKISIC MSLLGFLLSR LGLLLKVLLD WPVEVLYGAA ALNGLFGGFS AFWSGVMALG SLGSSEGRRS VRLILIDLML GLAGFCGSMA SGHLFKQMAG HSGQGLILTA CSVSCASFAL LYSLLVLKVP ESVAKPSQEL PAVDTVSGTV GTYRTLDPDQ LDQQYAVGHP PSPGKAKPHK TTIALLFVGA IIYDLAVVGT VDVIPLFVLR EPLGWNQVQV GYGMAAGYTI FITSFLGVLV FSRCFRDTTM IMIGMVSFGS GALLLAFVKE TYMFYIARAV MLFALIPVTT IRSAMSKLIK GSSYGKVFVI LQLSLALTGV VTSTLYNKIY QLTMDMFVGS CFALSSFLSF LAIIPISIVA YKQVPLSPYG DIIEK
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Attributs du produit: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

niveau d'endotoxine: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Commentaires:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: SLC46A2 (Solute Carrier Family 46 Member 2 (SLC46A2))

Autre désignation: SLC46A2 (SLC46A2 Produits)

Synonymes: Ly110 Protein, TSCOT Protein, 5430429N04Rik Protein, TSO-1C12 Protein, Tscot Protein, solute carrier family 46 member 2 Protein, solute carrier family 46, member 2 Protein, SLC46A2 Protein, Slc46a2 Protein

Sujet: Solute carrier family 46 member 2 (Thymic stromal cotransporter homolog),FUNCTION: Proton-coupled transporter that delivers pathogen-associated or danger-associated molecular patterns to cytosolic pattern recognition receptors as part of the innate immune response to microbes or tissue injury (PubMed:28539433, PubMed:34235268). Has selectivity toward muropeptides that contain the amino acid diaminopimelic acid (DAP-type peptidoglycan muropeptides) including Tri-DAP and tracheal toxin (TCT), common in Gram-negative bacteria and Gram-positive bacilli. In the context of immune recognition of skin microbiota, shuttles bacterial muropeptides across the endolysosomal membranes into the cytosol for recognition by NOD1, triggering MYD88-dependent secretion of IL1A and neutrophil recruitment in a pyroptosis-type inflammatory process (PubMed:28539433). To a lesser extent and redundantly, transports muramyl dipeptides derived from most bacterial proteoglycans, eliciting NOD2 receptor activation and downstream inflammatory responses (PubMed:28539433). Postulated to function as a dominant importer of cyclic GMP-AMP dinucleotides (cGAMPs) in monocyte and macrophage cell lineages. Selectively imports cGAMPs derived from pathogenic bacteria such as 3'3'-cGAMP thus providing for differential immune recognition of pathogenic versus commensal bacteria. During tumorigenesis may transport extracellular tumor-derived 2'3'-cGAMP across the plasma membrane of M1-polarized macrophages to activate the anti-tumoral stimulator of interferon genes (STING) pathway (PubMed:34235268). The transport mechanism, its electrogenicity and stoichiometry remain to be elucidated (Probable). {ECO:0000269|PubMed:28539433, ECO:0000269|PubMed:34235268, ECO:0000305}.

Poids moléculaire: 51.1 kDa

UniProt: Q9BY10