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COX2 Protein (AA 1-227) (rho-1D4 tag)

Crystallography grade COX2 Origine: Humain Hôte: Cellules d'insectes Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, ELISA, SDS, Crys
N° du produit ABIN3111472
  • Antigène Voir toutes COX2 Protéines
    COX2 (Cytochrome C Oxidase Subunit II (COX2))
    Type de proteíne
    Recombinant
    Attributs du protein
    AA 1-227
    Origine
    • 2
    • 2
    • 2
    • 1
    Humain
    Source
    • 4
    • 2
    • 1
    Cellules d'insectes
    Purification/Conjugué
    Cette COX2 protéine est marqué à la rho-1D4 tag.
    Application
    Western Blotting (WB), ELISA, SDS-PAGE (SDS), Crystallization (Crys)
    Séquence
    MAHAAQVGLQ DATSPIMEEL ITFHDHALMI IFLICFLVLY ALFLTLTTKL TNTNISDAQE METVWTILPA IILVLIALPS LRILYMTDEV NDPSLTIKSI GHQWYWTYEY TDYGGLIFNS YMLPPLFLEP GDLRLLDVDN RVVLPIEAPI RMMITSQDVL HSWAVPTLGL KTDAIPGRLN QTTFTATRPG VYYGQCSEIC GANHSFMPIV LELIPLKIFE MGPVFTL
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Attributs du produit
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Human MT-CO2 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Purification
    Three step purification of membrane proteins expressed in baculovirus infected SF9 insect cells:
    1. Membrane proteins are fractioned by ultracentrifugation and subsequently solubilized with different detergents (detergent screen). Samples are analyzed by Western blot.
    2. The best performing detergent is used for solubilization and the proteins are purified via their rho1D4 tag via two rho1D4 antibody columns: one DTT resistant, the other one not. Eluate fractions are analyzed by Western blot.
    3. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatograph. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Pureté
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Stérilité
    0.22 μm filtered
    niveau d'endotoxine
    Protein is endotoxin-free.
    Classe de qualité
    Crystallography grade
    Top Product
    Discover our top product COX2 Protéine
  • Indications d'application
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
    Commentaires

    In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Conseil sur la manipulation
    Avoid repeated freeze-thaw cycles.
    Stock
    -80 °C
    Stockage commentaire
    Store at -80°C.
    Date de péremption
    Unlimited (if stored properly)
  • Antigène
    COX2 (Cytochrome C Oxidase Subunit II (COX2))
    Autre désignation
    MT-CO2 (COX2 Produits)
    Synonymes
    COX-2 Protein, Cox2 Protein, COII Protein, MTCO2 Protein, cox Protein, SC6G10.29c Protein, cCOII Protein, prostaglandin-endoperoxide synthase 2 Protein, cytochrome c oxidase subunit II Protein, COXII Protein, cytochrome c oxidase subunit ii Protein, cytochrome C oxidase subunit II Protein, cytochrome c oxidase subunit 2 Protein, cytochrome oxidase subunit 2 Protein, Ptgs2 Protein, COX2 Protein, Smed_0518 Protein, Mrub_0709 Protein, Ocepr_1683 Protein, SCO2156 Protein, coxB Protein, Dtpsy_2860 Protein, Ndas_3150 Protein, Mesil_0628 Protein, Bresu_0801 Protein, cox2 Protein
    Sujet
    Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Subunits 1-3 form the functional core of the enzyme complex. Subunit 2 transfers the electrons from cytochrome c via its binuclear copper A center to the bimetallic center of the catalytic subunit 1.
    Poids moléculaire
    26.7 kDa Including tag.
    UniProt
    P00403
    Pathways
    Brown Fat Cell Differentiation, Positive Regulation of fat Cell Differentiation
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