JAG1 Protein (AA 34-1218) (rho-1D4 tag)

N° du produit ABIN3114168

Détail du produit

Antigène: JAG1 (Jagged 1 (JAG1))

Type de proteíne: Recombinant

Attributs du protein: AA 34-1218

Origine: Humain

Source: Cellules d'insectes

Purification/Conjugué: Cette JAG1 protéine est marqué à la rho-1D4 tag.

Application: SDS-PAGE (SDS), Western Blotting (WB), ELISA, Crystallization (Crys)

Séquence: QFELEILSMQ NVNGELQNGN CCGGARNPGD RKCTRDECDT YFKVCLKEYQ SRVTAGGPCS FGSGSTPVIG GNTFNLKASR GNDRNRIVLP FSFAWPRSYT LLVEAWDSSN DTVQPDSIIE KASHSGMINP SRQWQTLKQN TGVAHFEYQI RVTCDDYYYG FGCNKFCRPR DDFFGHYACD QNGNKTCMEG WMGPECNRAI CRQGCSPKHG SCKLPGDCRC QYGWQGLYCD KCIPHPGCVH GICNEPWQCL CETNWGGQLC DKDLNYCGTH QPCLNGGTCS NTGPDKYQCS CPEGYSGPNC EIAEHACLSD PCHNRGSCKE TSLGFECECS PGWTGPTCST NIDDCSPNNC SHGGTCQDLV NGFKCVCPPQ WTGKTCQLDA NECEAKPCVN AKSCKNLIAS YYCDCLPGWM GQNCDININD CLGQCQNDAS CRDLVNGYRC ICPPGYAGDH CERDIDECAS NPCLNGGHCQ NEINRFQCLC PTGFSGNLCQ LDIDYCEPNP CQNGAQCYNR ASDYFCKCPE DYEGKNCSHL KDHCRTTPCE VIDSCTVAMA SNDTPEGVRY ISSNVCGPHG KCKSQSGGKF TCDCNKGFTG TYCHENINDC ESNPCRNGGT CIDGVNSYKC ICSDGWEGAY CETNINDCSQ NPCHNGGTCR DLVNDFYCDC KNGWKGKTCH SRDSQCDEAT CNNGGTCYDE GDAFKCMCPG GWEGTTCNIA RNSSCLPNPC HNGGTCVVNG ESFTCVCKEG WEGPICAQNT NDCSPHPCYN SGTCVDGDNW YRCECAPGFA GPDCRININE CQSSPCAFGA TCVDEINGYR CVCPPGHSGA KCQEVSGRPC ITMGSVIPDG AKWDDDCNTC QCLNGRIACS KVWCGPRPCL LHKGHSECPS GQSCIPILDD QCFVHPCTGV GECRSSSLQP VKTKCTSDSY YQDNCANITF TFNKEMMSPG LTTEHICSEL RNLNILKNVS AEYSIYIACE PSPSANNEIH VAISAEDIRD DGNPIKEITD KIIDLVSKRD GNSSLIAAVA EVRVQRRPLK NRTDFLVPLL SSVLTVAWIC CLVTAFYWCL RKRRKPGSHT HSASEDNTTN NVREQLNQIK NPIEKHGANT VPIKDYENKN SKMSKIRTHN SEVEEDDMDK HQQKARFAKQ PAYTLVDREE KPPNGTPTKH PNWTNKQDNR DLESAQSLNR MEYIV
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Attributs du produit:

• Made in Germany - from design to production - by highly experienced protein experts.
• Human JAG1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Three step purification of membrane proteins expressed in baculovirus infected SF9 insect cells:

1. Membrane proteins are fractioned by ultracentrifugation and subsequently solubilized with different detergents (detergent screen). Samples are analyzed by Western blot.
2. The best performing detergent is used for solubilization and the proteins are purified via their rho1D4 tag via two rho1D4 antibody columns: one DTT resistant, the other one not. Eluate fractions are analyzed by Western blot.
3. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatograph. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Protein is endotoxin-free.

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Commentaires:

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: JAG1 (Jagged 1 (JAG1))

Autre désignation: JAG1 (JAG1 Produits)

Synonymes: AGS Protein, AHD Protein, AWS Protein, CD339 Protein, HJ1 Protein, JAGL1 Protein, ABE2 Protein, Gsfabe2 Protein, Htu Protein, Ozz Protein, Ser-1 Protein, JAG1 Protein, jag3 Protein, cb243 Protein, jag1 Protein, sb:cb243 Protein, serrateC Protein, jagged1 Protein, serrate Protein, serrate-1 Protein, x-serrate-1 Protein, jagged 1 Protein, jagged 1b Protein, jagged 1a Protein, jagged 1 L homeolog Protein, JAG1 Protein, Jag1 Protein, jag1 Protein, jag1b Protein, jag1a Protein, jag1.L Protein

Sujet: Ligand for multiple Notch receptors and involved in the mediation of Notch signaling. May be involved in cell-fate decisions during hematopoiesis. Seems to be involved in early and late stages of mammalian cardiovascular development. Inhibits myoblast differentiation (By similarity). Enhances fibroblast growth factor-induced angiogenesis (in vitro). {ECO:0000250, ECO:0000269|PubMed:18660822, ECO:0000269|PubMed:9462510}.

Poids moléculaire: 131.5 kDa Including tag.

UniProt: P78504

Pathways: Signalisation Notch, Stem Cell Maintenance