NOTCH2 Protein (AA 1697-2471) (rho-1D4 tag)

N° du produit ABIN3114343

Détail du produit

Antigène: NOTCH2 (Notch 2 (NOTCH2))

Type de proteíne: Recombinant

Attributs du protein: AA 1697-2471

Origine: Humain

Source: Cellules d'insectes

Purification/Conjugué: Cette NOTCH2 protéine est marqué à la rho-1D4 tag.

Application: Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)

Séquence: VIMAKRKRKH GSLWLPEGFT LRRDASNHKR REPVGQDAVG LKNLSVQVSE ANLIGTGTSE HWVDDEGPQP KKVKAEDEAL LSEEDDPIDR RPWTQQHLEA ADIRRTPSLA LTPPQAEQEV DVLDVNVRGP DGCTPLMLAS LRGGSSDLSD EDEDAEDSSA NIITDLVYQG ASLQAQTDRT GEMALHLAAR YSRADAAKRL LDAGADANAQ DNMGRCPLHA AVAADAQGVF QILIRNRVTD LDARMNDGTT PLILAARLAV EGMVAELINC QADVNAVDDH GKSALHWAAA VNNVEATLLL LKNGANRDMQ DNKEETPLFL AAREGSYEAA KILLDHFANR DITDHMDRLP RDVARDRMHH DIVRLLDEYN VTPSPPGTVL TSALSPVICG PNRSFLSLKH TPMGKKSRRP SAKSTMPTSL PNLAKEAKDA KGSRRKKSLS EKVQLSESSV TLSPVDSLES PHTYVSDTTS SPMITSPGIL QASPNPMLAT AAPPAPVHAQ HALSFSNLHE MQPLAHGAST VLPSVSQLLS HHHIVSPGSG SAGSLSRLHP VPVPADWMNR MEVNETQYNE MFGMVLAPAE GTHPGIAPQS RPPEGKHITT PREPLPPIVT FQLIPKGSIA QPAGAPQPQS TCPPAVAGPL PTMYQIPEMA RLPSVAFPTA MMPQQDGQVA QTILPAYHPF PASVGKYPTP PSQHSYASSN AAERTPSHSG HLQGEHPYLT PSPESPDQWS SSSPHSASDW SDVTTSPTPG GAGGGQRGPG THMSEPPHNN MQVYA
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Attributs du produit:

• Made in Germany - from design to production - by highly experienced protein experts.
• Human NOTCH2 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Three step purification of membrane proteins expressed in baculovirus infected SF9 insect cells:

1. Membrane proteins are fractioned by ultracentrifugation and subsequently solubilized with different detergents (detergent screen). Samples are analyzed by Western blot.
2. The best performing detergent is used for solubilization and the proteins are purified via their rho1D4 tag via two rho1D4 antibody columns: one DTT resistant, the other one not. Eluate fractions are analyzed by Western blot.
3. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatograph. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Protein is endotoxin-free.

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Commentaires:

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: NOTCH2 (Notch 2 (NOTCH2))

Autre désignation: NOTCH2 (NOTCH2 Produits)

Synonymes: AGS2 Protein, HJCYS Protein, hN2 Protein, AI853703 Protein, N2 Protein, notch6 Protein, sb:cb884 Protein, neurogenic locus notch homolog protein 2 Protein, notch 2 Protein, notch 3 Protein, LOC581186 Protein, NOTCH2 Protein, notch3 Protein, Notch2 Protein, notch2 Protein

Sujet: Functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs (By similarity). Involved in bone remodeling and homeostasis. In collaboration with RELA/p65 enhances NFATc1 promoter activity and positively regulates RANKL-induced osteoclast differentiation. Positively regulates self-renewal of liver cancer cells (PubMed:25985737). {ECO:0000250|UniProtKB:O35516, ECO:0000269|PubMed:21378985, ECO:0000269|PubMed:21378989, ECO:0000269|PubMed:25985737}.

Poids moléculaire: 84.4 kDa Including tag.

UniProt: Q04721

Pathways: Signalisation Notch, Stem Cell Maintenance