RNF43 Protein (AA 24-783) (rho-1D4 tag)

N° du produit ABIN3115590

Détail du produit

Antigène: RNF43 (Ring Finger Protein 43 (RNF43))

Type de proteíne: Recombinant

Attributs du protein: AA 24-783

Origine: Humain

Source: Cellules d'insectes

Purification/Conjugué: Cette RNF43 protéine est marqué à la rho-1D4 tag.

Application: Crystallization (Crys), ELISA, SDS-PAGE (SDS), Western Blotting (WB)

Séquence: GFGRTGLVLA AAVESERSAE QKAIIRVIPL KMDPTGKLNL TLEGVFAGVA EITPAEGKLM QSHPLYLCNA SDDDNLEPGF ISIVKLESPR RAPRPCLSLA SKARMAGERG ASAVLFDITE DRAAAEQLQQ PLGLTWPVVL IWGNDAEKLM EFVYKNQKAH VRIELKEPPA WPDYDVWILM TVVGTIFVII LASVLRIRCR PRHSRPDPLQ QRTAWAISQL ATRRYQASCR QARGEWPDSG SSCSSAPVCA ICLEEFSEGQ ELRVISCLHE FHRNCVDPWL HQHRTCPLCM FNITEGDSFS QSLGPSRSYQ EPGRRLHLIR QHPGHAHYHL PAAYLLGPSR SAVARPPRPG PFLPSQEPGM GPRHHRFPRA AHPRAPGEQQ RLAGAQHPYA QGWGLSHLQS TSQHPAACPV PLRRARPPDS SGSGESYCTE RSGYLADGPA SDSSSGPCHG SSSDSVVNCT DISLQGVHGS SSTFCSSLSS DFDPLVYCSP KGDPQRVDMQ PSVTSRPRSL DSVVPTGETQ VSSHVHYHRH RHHHYKKRFQ WHGRKPGPET GVPQSRPPIP RTQPQPEPPS PDQQVTRSNS AAPSGRLSNP QCPRALPEPA PGPVDASSIC PSTSSLFNLQ KSSLSARHPQ RKRRGGPSEP TPGSRPQDAT VHPACQIFPH YTPSVAYPWS PEAHPLICGP PGLDKRLLPE TPGPCYSNSQ PVWLCLTPRQ PLEPHPPGEG PSEWSSDTAE GRPCPYPHCQ VLSAQPGSEE ELEELCEQAV
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Attributs du produit:

• Made in Germany - from design to production - by highly experienced protein experts.
• Human RNF43 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Three step purification of membrane proteins expressed in baculovirus infected SF9 insect cells:

1. Membrane proteins are fractioned by ultracentrifugation and subsequently solubilized with different detergents (detergent screen). Samples are analyzed by Western blot.
2. The best performing detergent is used for solubilization and the proteins are purified via their rho1D4 tag via two rho1D4 antibody columns: one DTT resistant, the other one not. Eluate fractions are analyzed by Western blot.
3. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatograph. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Protein is endotoxin-free.

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Commentaires:

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: RNF43 (Ring Finger Protein 43 (RNF43))

Autre désignation: RNF43 (RNF43 Produits)

Synonymes: RNF124 Protein, URCC Protein, 4732452J19Rik Protein, RGD1305204 Protein, ring finger protein 43 Protein, RNF43 Protein, Rnf43 Protein

Sujet: E3 ubiquitin-protein ligase that acts as a negative regulator of the Wnt signaling pathway by mediating the ubiquitination, endocytosis and subsequent degradation of Wnt receptor complex components Frizzled. Acts on both canonical and non-canonical Wnt signaling pathway. Acts as a tumor suppressor in the intestinal stem cell zone by inhibiting the Wnt signaling pathway, thereby resticting the size of the intestinal stem cell zone. {ECO:0000269|PubMed:18313049, ECO:0000269|PubMed:22575959, ECO:0000269|PubMed:22895187}.

Poids moléculaire: 84.4 kDa Including tag.

UniProt: Q68DV7

Pathways: Signalisation WNT