Corin Protein (AA 1-1042) (Strep Tag)

N° du produit ABIN3119498

Détail du produit

Antigène: Corin (CORIN) (Corin, Serine Peptidase (CORIN))

Type de proteíne: Recombinant

Attributs du protein: AA 1-1042

Origine: Humain

Source: Tobacco (Nicotiana tabacum)

Purification/Conjugué: Cette Corin protéine est marqué à la Strep Tag.

Application: Western Blotting (WB), SDS-PAGE (SDS), ELISA

Séquence: MKQSPALAPE ERCRRAGSPK PVLRADDNNM GNGCSQKLAT ANLLRFLLLV LIPCICALVL LLVILLSYVG TLQKVYFKSN GSEPLVTDGE IQGSDVILTN TIYNQSTVVS TAHPDQHVPA WTTDASLPGD QSHRNTSACM NITHSQCQML PYHATLTPLL SVVRNMEMEK FLKFFTYLHR LSCYQHIMLF GCTLAFPECI IDGDDSHGLL PCRSFCEAAK EGCESVLGMV NYSWPDFLRC SQFRNQTESS NVSRICFSPQ QENGKQLLCG RGENFLCASG ICIPGKLQCN GYNDCDDWSD EAHCNCSENL FHCHTGKCLN YSLVCDGYDD CGDLSDEQNC DCNPTTEHRC GDGRCIAMEW VCDGDHDCVD KSDEVNCSCH SQGLVECRNG QCIPSTFQCD GDEDCKDGSD EENCSVIQTS CQEGDQRCLY NPCLDSCGGS SLCDPNNSLN NCSQCEPITL ELCMNLPYNS TSYPNYFGHR TQKEASISWE SSLFPALVQT NCYKYLMFFS CTILVPKCDV NTGEHIPPCR ALCEHSKERC ESVLGIVGLQ WPEDTDCSQF PEENSDNQTC LMPDEYVEEC SPSHFKCRSG QCVLASRRCD GQADCDDDSD EENCGCKERD LWECPSNKQC LKHTVICDGF PDCPDYMDEK NCSFCQDDEL ECANHACVSR DLWCDGEADC SDSSDEWDCV TLSINVNSSS FLMVHRAATE HHVCADGWQE ILSQLACKQM GLGEPSVTKL IQEQEKEPRW LTLHSNWESL NGTTLHELLV NGQSCESRSK ISLLCTKQDC GRRPAARMNK RILGGRTSRP GRWPWQCSLQ SEPSGHICGC VLIAKKWVLT VAHCFEGREN AAVWKVVLGI NNLDHPSVFM QTRFVKTIIL HPRYSRAVVD YDISIVELSE DISETGYVRP VCLPNPEQWL EPDTYCYITG WGHMGNKMPF KLQEGEVRII SLEHCQSYFD MKTITTRMIC AGYESGTVDS CMGDSGGPLV CEKPGGRWTL FGLTSWGSVC FSKVLGPGVY SNVSYFVEWI KRQIYIQTFL LN
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Attributs du produit: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

niveau d'endotoxine: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Commentaires:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: Corin (CORIN) (Corin, Serine Peptidase (CORIN))

Autre désignation: CORIN (CORIN Produits)

Synonymes: CORIN Protein, CG2105 Protein, Dmel\\CG2105 Protein, SP75 Protein, LOC559109 Protein, AV273130 Protein, Lrp4 Protein, ATC2 Protein, CRN Protein, PEE5 Protein, TMPRSS10 Protein, corin, serine peptidase Protein, CG2105 gene product from transcript CG2105-RC Protein, novel protein similar to H.sapiens CORIN, corin, serine peptidase (CORIN) Protein, corin Protein, CORIN Protein, Corin Protein, LOC559109 Protein

Sujet: Atrial natriuretic peptide-converting enzyme (EC 3.4.21.-) (Corin) (Heart-specific serine proteinase ATC2) (Pro-ANP-converting enzyme) (Transmembrane protease serine 10) [Cleaved into: Atrial natriuretic peptide-converting enzyme, N-terminal propeptide, Atrial natriuretic peptide-converting enzyme, activated protease fragment, Atrial natriuretic peptide-converting enzyme, 180 kDa soluble fragment, Atrial natriuretic peptide-converting enzyme, 160 kDa soluble fragment, Atrial natriuretic peptide-converting enzyme, 100 kDa soluble fragment],FUNCTION: Serine-type endopeptidase involved in atrial natriuretic peptide (NPPA) and brain natriuretic peptide (NPPB) processing (PubMed:10880574, PubMed:21288900, PubMed:20489134, PubMed:21763278). Converts through proteolytic cleavage the non-functional propeptides NPPA and NPPB into their active hormones, ANP and BNP(1-32) respectively, thereby regulating blood pressure in the heart and promoting natriuresis, diuresis and vasodilation (PubMed:10880574, PubMed:21288900, PubMed:20489134, PubMed:21763278). Proteolytic cleavage of pro-NPPA also plays a role in female pregnancy by promoting trophoblast invasion and spiral artery remodeling in uterus (PubMed:22437503). Also acts as a regulator of sodium reabsorption in kidney (By similarity). {ECO:0000250|UniProtKB:Q9Z319, ECO:0000269|PubMed:10880574, ECO:0000269|PubMed:20489134, ECO:0000269|PubMed:21288900, ECO:0000269|PubMed:21763278, ECO:0000269|PubMed:22437503}., FUNCTION: [Isoform 2]: Has weaker endopeptidase activity compared to isoform 1.

Poids moléculaire: 116.5 kDa

UniProt: Q9Y5Q5

Pathways: Regulation of Systemic Arterial Blood Pressure by Hormones