ARPC1A Protein (AA 1-370) (Strep Tag)

N° du produit ABIN3119892

Détail du produit

Antigène: ARPC1A (Actin Related Protein 2/3 Complex, Subunit 1A, 41kDa (ARPC1A))

Type de proteíne: Recombinant

Attributs du protein: AA 1-370

Origine: Souris

Source: Tobacco (Nicotiana tabacum)

Purification/Conjugué: Cette ARPC1A protéine est marqué à la Strep Tag.

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Séquence: MSLHQFLLEP ITCHAWNRDR TQIALSPNNH EVHIYKKNGS QWTKAHELKE HNGHITGIDW APKSDRIVTC GADRNAYVWS QKDGIWKPTL VILRINRAAT FVKWSPLENK FAVGSGARLI SVCYFESEND WWVSKHIKKP IRSTVLSLDW HPNNVLLAAG SCDFKCRVFS AYIKEVDEKP ASTPWGSKMP FGQLMSEFGG SGTGGWVHGV SFSASGNRLA WVSHDSTVSV ADASKSVQVS TLRTEFLPLL SVSFVSENSV VAAGHDCCPM LFNYDDRGCL TFVSKLDVPK QSIQRNMSAM ERFRNMDKRA TTEDRNTALE TLHQNSITQV SIYEVDKQDC RKFCTTGIDG AMTIWDFKTL ESSIQGLRIM
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Attributs du produit: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

niveau d'endotoxine: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Commentaires:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: ARPC1A (Actin Related Protein 2/3 Complex, Subunit 1A, 41kDa (ARPC1A))

Autre désignation: Arpc1a (ARPC1A Produits)

Synonymes: Arc40 Protein, SOP2Hs Protein, SOP2L Protein, 0610010H08Rik Protein, 1110030K07Rik Protein, 41kDa Protein, AA407347 Protein, Sid32 Protein, Sid329 Protein, arpc1a Protein, MGC52839 Protein, MGC130836 Protein, wu:fk93e03 Protein, MGC76015 Protein, ARPC1A Protein, ACTIN-RELATED PROTEIN C1 Protein, ARPC1 Protein, F7F1.12 Protein, F7F1_12 Protein, actin-related protein C1A Protein, actin related protein 2/3 complex subunit 1A Protein, actin related protein 2/3 complex, subunit 1A Protein, actin related protein 2/3 complex subunit 1A S homeolog Protein, actin-related protein C1A Protein, actin related protein 2/3 complex, subunit 1A, 41kDa Protein, actin-related protein 2/3 complex subunit 1A-like Protein, ARPC1A Protein, Arpc1a Protein, arpc1a.S Protein, arpc1a Protein, LOC100352239 Protein

Sujet: Actin-related protein 2/3 complex subunit 1A (SOP2-like protein) (Sid 329),FUNCTION: Probably functions as a component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks (By similarity). In addition to its role in the cytoplasmic cytoskeleton, the Arp2/3 complex also promotes actin polymerization in the nucleus, thereby regulating gene transcription and repair of damaged DNA (By similarity). {ECO:0000250|UniProtKB:Q8AVT9, ECO:0000250|UniProtKB:Q92747}.

Poids moléculaire: 41.6 kDa

UniProt: Q9R0Q6

Pathways: Regulation of Actin Filament Polymerization