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MMP17 Protein (AA 125-558) (His tag)

Crystallography grade MMP17 Origine: Souris Hôte: Cellules d'insectes Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, Crys, ELISA
N° du produit ABIN3119898
  • Antigène Voir toutes MMP17 Protéines
    MMP17 (Matrix Metallopeptidase 17 (Membrane-inserted) (MMP17))
    Type de proteíne
    Recombinant
    Attributs du protein
    AA 125-558
    Origine
    Souris
    Source
    • 2
    • 1
    Cellules d'insectes
    Purification/Conjugué
    Cette MMP17 protéine est marqué à la His tag.
    Application
    SDS-PAGE (SDS), Western Blotting (WB), Crystallization (Crys), ELISA
    Séquence
    QTPPPTKWSK RNLSWRVRTF PRDSPLGRDT VRALMYYALK VWSDITPLNF HEVAGNAADI QIDFSKADHN DGYPFDGPGG TVAHAFFPGD HHTAGDTHFD DDEPWTFRSS DAHGMDLFAV AVHEFGHAIG LSHVAAPSSI MQPYYQGPVG DPLRYGLPYE DRVRVWQLYG VRESVSPTAQ LDTPEPEEPP LLPEPPNNRS STPPQKDVPH RCTAHFDAVA QIRGEAFFFK GKYFWRLTRD RHLVSLQPAQ MHRFWRGLPL HLDSVDAVYE RTSDHKIVFF KGDRYWVFKD NNVEEGYPRP VSDFSLPPGG IDAVFSWAHN DRTYFFKDQL YWRYDDHTRR MDPGYPAQGP LWRGVPSMLD DAMRWSDGAS YFFRGQEYWK VLDGELEAAP GYPQSTARDW LVCGEPLADA EDVGPGPQGR SGAQDGLAVC SCTS
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Attributs du produit
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Mouse Mmp17 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Purification
    Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Pureté
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Stérilité
    0.22 μm filtered
    niveau d'endotoxine
    Protein is endotoxin free.
    Classe de qualité
    Crystallography grade
    Top Product
    Discover our top product MMP17 Protéine
  • Indications d'application
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    Commentaires

    Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Conseil sur la manipulation
    Avoid repeated freeze-thaw cycles.
    Stock
    -80 °C
    Stockage commentaire
    Store at -80°C.
    Date de péremption
    Unlimited (if stored properly)
  • Antigène
    MMP17 (Matrix Metallopeptidase 17 (Membrane-inserted) (MMP17))
    Autre désignation
    Mmp17 (MMP17 Produits)
    Synonymes
    MT4-MMP Protein, CBTF Protein, DRBF Protein, DRBP76 Protein, MMP4 Protein, MPHOSPH4 Protein, MPP4 Protein, NF-AT-90 Protein, NF110 Protein, NF110b Protein, NF90 Protein, NF90a Protein, NF90b Protein, NFAR Protein, NFAR-1 Protein, NFAR2 Protein, TCP110 Protein, TCP80 Protein, MT4MMP Protein, MTMMP4 Protein, mmp17 Protein, matrix metallopeptidase 17 Protein, interleukin enhancer binding factor 3 Protein, matrix metallopeptidase 17a Protein, MMP17 Protein, ILF3 Protein, Mmp17 Protein, mmp17a Protein
    Sujet
    Endopeptidase that degrades various components of the extracellular matrix, such as fibrin. May be involved in the activation of membrane-bound precursors of growth factors or inflammatory mediators, such as tumor necrosis factor-alpha. May also be involved in tumoral process. Not obvious if able to proteolytically activate progelatinase A. Does not hydrolyze collagen types I, II, III, IV and V, gelatin, fibronectin, laminin, decorin nor alpha1-antitrypsin. {ECO:0000269|PubMed:10799478}.
    Poids moléculaire
    50.2 kDa Including tag.
    UniProt
    Q9R0S3
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