Nerve Growth Factor Receptor (TNFRSF16) Associated Protein 1 (NGFRAP1) (AA 1-124) protein (Strep Tag)

N° du produit ABIN3120034

Détail du produit

Antigène: Nerve Growth Factor Receptor (TNFRSF16) Associated Protein 1 (NGFRAP1)

Type de proteíne: Recombinant

Attributs du protein: AA 1-124

Origine: Souris

Source: Tobacco (Nicotiana tabacum)

Purification/Conjugué: Strep Tag

Application: ELISA, SDS-PAGE (SDS), Western Blotting (WB)

Séquence: MANVHQENEE MEQPLQNGQE DRPVGGGEGH QPAANNNNNN HNHNHNHHRR GQARRLAPNF RWAIPNRQMN DGLGGDGDDM EMFMEEMREI RRKLRELQLR NCLRILMGEL SNHHDHHDEF CLMP
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Attributs du produit: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

niveau d'endotoxine: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Commentaires:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: Nerve Growth Factor Receptor (TNFRSF16) Associated Protein 1 (NGFRAP1)

Autre désignation: Bex3 (NGFRAP1 Produits)

Synonymes: BEX3 Protein, Bex Protein, DXS6984E Protein, HGR74 Protein, NADE Protein, NGFRAP1 Protein, LNGFR Protein, Tnfrsf16 Protein, p75 Protein, p75NGFR Protein, p75NTR Protein, AL033356 Protein, Bex3 Protein, DXWsu67e Protein, Gcap27 Protein, Nade Protein, WBP5 Protein, brain expressed X-linked 3 Protein, nerve growth factor receptor (TNFR superfamily, member 16) Protein, BEX3 Protein, Ngfr Protein, Bex3 Protein

Sujet: Protein BEX3 (Brain-expressed X-linked protein 3 homolog) (Nerve growth factor receptor-associated protein 1) (p75NTR-associated cell death executor),FUNCTION: May be a signaling adapter molecule involved in NGFR/p75NTR-mediated apoptosis induced by NGF (PubMed:10764727). Plays a role in zinc-triggered neuronal death (PubMed:10764727). In absence of reductive stress, acts as a pseudosubstrate for the CRL2(FEM1B) complex: associates with FEM1B via zinc, thereby preventing association between FEM1B and its substrates (PubMed:34562363). {ECO:0000269|PubMed:10764727, ECO:0000269|PubMed:34562363}.

Poids moléculaire: 14.5 kDa

UniProt: Q9WTZ9

Pathways: Neurotrophin Signaling Pathway, Positive Regulation of Endopeptidase Activity