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ORC5 Protein (AA 1-435) (His tag)

Crystallography grade ORC5 Origine: Souris Hôte: Cellules d'insectes Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, Crys, SDS
N° du produit ABIN3120101
  • Antigène Voir toutes ORC5 Protéines
    ORC5 (Origin Recognition Complex, Subunit 5 (ORC5))
    Type de proteíne
    Recombinant
    Attributs du protein
    AA 1-435
    Origine
    • 2
    • 1
    • 1
    Souris
    Source
    • 2
    • 1
    • 1
    Cellules d'insectes
    Purification/Conjugué
    Cette ORC5 protéine est marqué à la His tag.
    Application
    ELISA, Western Blotting (WB), Crystallization (Crys), SDS-PAGE (SDS)
    Séquence
    MSHLESMVLC REAQVSTLQS LFGERHHFSF PSIFIYGHTA SGKTYVTQTL LKTLELPHAF VNCVECFTSR LLLEQILNKL SHLNSSDAGC STEMTCETFN DFVQLFKQVT SAEHLKDQTV YIVLDKAEYL RDMEANLLPG LLRLQELTDR NVTVIFLSEI IWEKFRPNTG CFEPFVLYFP DYSIGNLQKI LSHDHPPEYS ADFYAAYINI LLGVFYTVCR DLKELRHLAV LNFPKYCEPV VKGEAGERDT RKLWRNIEPH LKKAMQTVYL REISSSQWEK LQKDNTDPGQ LKGLSAYTHM ELPYYSKFIL IAAYLASYNP ARTDKRFFLK HHGKIKKTNF LKKHEKTSNH LLGPKPFPLD RLLAILYSIV DSRVAPTANI FSQITSLVTL QLLTIVGHED QLNGPKYKCT VSLDFIRAIA RMVNFDIIKY LYDFL
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Attributs du produit
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Mouse Orc5 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Purification
    Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Pureté
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Stérilité
    0.22 μm filtered
    niveau d'endotoxine
    Protein is endotoxin free.
    Classe de qualité
    Crystallography grade
    Top Product
    Discover our top product ORC5 Protéine
  • Indications d'application
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    Commentaires

    Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Conseil sur la manipulation
    Avoid repeated freeze-thaw cycles.
    Stock
    -80 °C
    Stockage commentaire
    Store at -80°C.
    Date de péremption
    Unlimited (if stored properly)
  • Antigène
    ORC5 (Origin Recognition Complex, Subunit 5 (ORC5))
    Autre désignation
    Orc5 (ORC5 Produits)
    Synonymes
    Orc5l Protein, BG:DS00941.8 Protein, CG7833 Protein, D-orc5 Protein, DmORC5 Protein, DmOrc5 Protein, Dmel\\CG7833 Protein, ORC Protein, ORC5 Protein, br39 Protein, dORC5 Protein, l(2)34Df Protein, l(2)br39 Protein, orc5 Protein, orc5l Protein, wu:fb62d08 Protein, zgc:77827 Protein, ORC5L Protein, DDBDRAFT_0191826 Protein, DDBDRAFT_0233112 Protein, DDB_0191826 Protein, DDB_0233112 Protein, ORC5P Protein, ORC5T Protein, AL033327 Protein, MmORC5 Protein, origin recognition complex, subunit 5 Protein, Origin recognition complex subunit 5 Protein, origin recognition complex subunit 5 Protein, Orc5 Protein, orc5 Protein, ORC5 Protein, orcE Protein
    Sujet
    Component of the origin recognition complex (ORC) that binds origins of replication. DNA-binding is ATP-dependent. The specific DNA sequences that define origins of replication have not been identified yet. ORC is required to assemble the pre-replication complex necessary to initiate DNA replication (By similarity). {ECO:0000250}.
    Poids moléculaire
    51.2 kDa Including tag.
    UniProt
    Q9WUV0
    Pathways
    Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA
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