Melanocortin 4 Receptor (MC4R) (AA 1-332) protein (rho-1D4 tag)

Détails pour le produit réf. ABIN3120493, Fournisseur: Connectez-vous pour afficher
Nom de la protéine
  • zMC4
  • si:dkey-151m6.62
  • mc4r
  • MC4R
  • LOC100218148
  • Mc4-r
  • melanocortin 4 receptor
  • mc4r
  • MC4R
  • Mc4r
Attributs du protein
AA 1-332
1
1
Origine
Souris
5
1
1
Source
Cellules d'insectes
2
1
1
1
Type de proteíne
Recombinant
Purification/Conjugué
rho-1D4 tag
Application
Crystallization (Crys), ELISA, SDS-PAGE (SDS), Western Blotting (WB)
Options
Fournisseur
Connectez-vous pour afficher
N° du produit (Fournisseur)
Connectez-vous pour afficher
Séquence MNSTHHHGMY TSLHLWNRSS YGLHGNASES LGKGHPDGGC YEQLFVSPEV FVTLGVISLL ENILVIVAIA KNKNLHSPMY FFICSLAVAD MLVSVSNGSE TIVITLLNST DTDAQSFTVN IDNVIDSVIC SSLLASICSL LSIAVDRYF TIFYALQYHN IMTVRRVGII ISCIWAACTV SGVLFIIYSD SSAVIICLIS MFFTMLVLMA SLYVHMFLMA RLHIKRIAVL PGTGTIRQGT NMKGAITLT ILIGVFVVCW APFFLHLLF YISCPQNPY CVCFMSHFNL YLILIMCNAV IDPLIYALRS QELRKTFKEI ICFYPLGGIC ELSSRYENLY FQGGSSGTET SQVAPA
Sequence with tag.
Attributs du produit
  • Made in Germany - from design to production - by highly experienced protein experts.
  • Mouse Mc4r Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
  • State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

Purification Three step purification of membrane proteins expressed in baculovirus infected SF9 insect cells:
  1. Membrane proteins are fractioned by ultracentrifugation and subsequently solubilized with different detergents (detergent screen). Samples are analyzed by Western blot.
  2. The best performing detergent is used for solubilization and the proteins are purified via their rho1D4 tag via two rho1D4 antibody columns: one DTT resistant, the other one not. Eluate fractions are analyzed by Western blot.
  3. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatograph. Eluate fractions are analyzed by SDS-PAGE and Western blot.
Pureté >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
Stérilité 0.22 μm filtered
niveau d'endotoxine Protein is endotoxin-free.
Classe de qualité Crystallography grade
Nom de la protéine
Sujet Receptor specific to the heptapeptide core common to adrenocorticotropic hormone and alpha-, beta-, and gamma-MSH. Plays a central role in energy homeostasis and somatic growth. This receptor is mediated by G proteins that stimulate adenylate cyclase (cAMP). {ECO:0000269|PubMed:23869016}.
Poids moléculaire 38.1 kDa Including tag.
UniProt P56450
Pathways Hormone Transport, cAMP Metabolic Process, Feeding Behaviour
Indications d'application In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
Commentaires

C-terminal Rho-tag

Restrictions For Research Use only
Format Liquid
Buffer 20 mM Hepes, pH 7.0, 100 mM NaCl, 2 mM EDTA, 0,1 % DM
Conseil sur la manipulation Avoid repeated freeze-thaw cycles.
Stock -80 °C
Stockage commentaire Store at -80°C.
Date de péremption Unlimited (if stored properly)
Images (Fournisseur)
 image for Melanocortin 4 Receptor (MC4R) (AA 1-332) protein (rho-1D4 tag) (ABIN3120493) „Crystallography Grade“ protein due to multi-step, protein-specific purification process
Western Blotting (WB) image for Melanocortin 4 Receptor (MC4R) (AA 1-332) protein (rho-1D4 tag) (ABIN3120493) Mouse Melanocortin 4 Receptor (MC4R) (AA 1-332), Fraction 11-13
 image for Melanocortin 4 Receptor (MC4R) (AA 1-332) protein (rho-1D4 tag) (ABIN3120493) Mouse Melanocortin 4 Receptor (MC4R) (AA 1-332), Gel filtration Superose 6, Fraction ...
Avez-vous cherché autre chose?