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ARL4C Protein (AA 2-192) (His tag)

Crystallography grade ARL4C Origine: Souris Hôte: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, Crys, SDS
N° du produit ABIN3120691
  • Antigène Voir toutes ARL4C Protéines
    ARL4C (ADP-Ribosylation Factor-Like 4C (ARL4C))
    Type de proteíne
    Recombinant
    Attributs du protein
    AA 2-192
    Origine
    Souris
    Source
    • 1
    • 1
    • 1
    Escherichia coli (E. coli)
    Purification/Conjugué
    Cette ARL4C protéine est marqué à la His tag.
    Application
    ELISA, Western Blotting (WB), Crystallization (Crys), SDS-PAGE (SDS)
    Séquence
    GNISSNISAF QSLHIVMLGL DSAGKTTVLY RLKFNEFVNT VPTIGFNTEK IKLSNGTAKG ISCHFWDVGG QEKLRPLWKS YSRCTDGIIY VVDSVDVDRL EEAKTELHKV TKFAENQGTP LLVIANKQDL PKSLPVAEIE KQLALHELIP ATTYHVQPAC AIIGEGLTEG MDKLYEMILK RRKSLKQKKK R
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Attributs du produit
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Mouse Arl4c Protein (raised in E. Coli) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Purification
    Two step purification of proteins expressed in bacterial culture:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Pureté
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Stérilité
    0.22 μm filtered
    niveau d'endotoxine
    Endotoxin has not been removed. Please contact us if you require endotoxin removal.
    Classe de qualité
    Crystallography grade
    Top Product
    Discover our top product ARL4C Protéine
  • Indications d'application
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    Commentaires

    Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Conseil sur la manipulation
    Avoid repeated freeze-thaw cycles.
    Stock
    -80 °C
    Stockage commentaire
    Store at -80°C.
    Date de péremption
    Unlimited (if stored properly)
  • Antigène
    ARL4C (ADP-Ribosylation Factor-Like 4C (ARL4C))
    Autre désignation
    Arl4c (ARL4C Produits)
    Synonymes
    ARL7 Protein, LAK Protein, A630084M22Rik Protein, Arl7 Protein, ARL4C Protein, MGC131112 Protein, arl7 Protein, fc01g11 Protein, fj62h11 Protein, zgc:77633 Protein, wu:fc01g11 Protein, wu:fj62h11 Protein, Arl4c-ps1 Protein, si:ch211-246m6.1 Protein, zgc:112100 Protein, ADP ribosylation factor like GTPase 4C Protein, ADP-ribosylation factor-like 4C Protein, ADP ribosylation factor like GTPase 4C L homeolog Protein, ADP-ribosylation factor-like protein 4C Protein, ADP-ribosylation factor-like 4Cb Protein, ADP-ribosylation factor like GTPase 4C Protein, ADP-ribosylation factor-like 4Ca Protein, ARL4C Protein, Arl4c Protein, arl4c.L Protein, arl4c Protein, arl4cb Protein, arl4ca Protein
    Sujet
    Small GTP-binding protein which cycles between an inactive GDP-bound and an active GTP-bound form, and the rate of cycling is regulated by guanine nucleotide exchange factors (GEF) and GTPase-activating proteins (GAP). GTP-binding protein that does not act as an allosteric activator of the cholera toxin catalytic subunit. May be involved in transport between a perinuclear compartment and the plasma membrane, apparently linked to the ABCA1-mediated cholesterol secretion pathway. Recruits CYTH1, CYTH2, CYTH3 and CYTH4 to the plasma membrane in the GDP-bound form. Regulates the microtubule-dependent intracellular vesicular transport from early endosome to recycling endosome process (By similarity). {ECO:0000250}.
    Poids moléculaire
    22.3 kDa Including tag.
    UniProt
    P61208
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