CDK5R1 Protein (AA 99-307) (His tag)

N° du produit ABIN3120721

Détail du produit

Antigène: CDK5R1 (Cyclin-Dependent Kinase 5, Regulatory Subunit 1 (p35) (CDK5R1))

Type de proteíne: Recombinant

Attributs du protein: AA 99-307

Origine: Souris

Source: Cellules d'insectes

Purification/Conjugué: Cette CDK5R1 protéine est marqué à la His tag.

Application: Western Blotting (WB), ELISA, SDS-PAGE (SDS), Crystallization (Crys)

Séquence: AQPPPAQPPA PPASQLSGSQ TGVSSSVKKA PHPAITSAGT PKRVIVQAST SELLRCLGEF LCRRCYRLKH LSPTDPVLWL RSVDRSLLLQ GWQDQGFITP ANVVFLYMLC RDVISSEVGS DHELQAVLLT CLYLSYSYMG NEISYPLKPF LVESCKEAFW DRCLSVINLM SSKMLQINAD PHYFTQVFSD LKNESGQEDK KRLLLGLDR
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Attributs du produit:

• Made in Germany - from design to production - by highly experienced protein experts.
• Mouse Cdk5r1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Protein is endotoxin free.

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Commentaires:

Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: CDK5R1 (Cyclin-Dependent Kinase 5, Regulatory Subunit 1 (p35) (CDK5R1))

Autre désignation: Cdk5r1 (CDK5R1 Produits)

Synonymes: CDK5P35 Protein, CDK5R Protein, NCK5A Protein, p23 Protein, p25 Protein, p35 Protein, p35nck5a Protein, cdk5r1 Protein, zgc:92814 Protein, CDK5R1 Protein, Cdk5r Protein, P25/P35 Protein, D11Bwg0379e Protein, Xp35.1 Protein, cyclin dependent kinase 5 regulatory subunit 1 Protein, cyclin-dependent kinase 5, regulatory subunit 1b (p35) Protein, cyclin-dependent kinase 5 regulatory subunit 1 Protein, S100 calcium binding protein A9 Protein, cyclin-dependent kinase 5, regulatory subunit 1 (p35) Protein, cyclin-dependent kinase 5, regulatory subunit 1 (p35) L homeolog Protein, CDK5R1 Protein, cdk5r1b Protein, Cdk5r1 Protein, S100A9 Protein, cdk5r1.L Protein

Classe de substances: Viral Protein

Sujet: P35 is a neuron specific activator of CDK5. The complex p35/CDK5 is required for neurite outgrowth and cortical lamination. Involved in dendritic spine morphogenesis by mediating the EFNA1-EPHA4 signaling. Activator of TPKII. The complex p35/CDK5 participates in the regulation of the circadian clock by modulating the function of CLOCK protein: phosphorylates CLOCK at 'Thr-451' and 'Thr-461' and regulates the transcriptional activity of the CLOCK-ARNTL/BMAL1 heterodimer in association with altered stability and subcellular distribution. {ECO:0000269|PubMed:17143272, ECO:0000269|PubMed:24235147}.

Poids moléculaire: 24.3 kDa Including tag.

UniProt: P61809

Pathways: Stem Cell Maintenance, Regulation of Cell Size, Positive Regulation of Endopeptidase Activity