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CRABP1 Protein (AA 2-137) (His tag)

Crystallography grade CRABP1 Origine: Souris Hôte: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
N° du produit ABIN3120814
  • Antigène Voir toutes CRABP1 Protéines
    CRABP1 (Cellular Retinoic Acid Binding Protein 1 (CRABP1))
    Type de proteíne
    Recombinant
    Attributs du protein
    AA 2-137
    Origine
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Souris
    Source
    • 5
    • 2
    • 1
    Escherichia coli (E. coli)
    Purification/Conjugué
    Cette CRABP1 protéine est marqué à la His tag.
    Application
    SDS-PAGE (SDS), Western Blotting (WB), ELISA, Crystallization (Crys)
    Séquence
    PNFAGTWKMR SSENFDELLK ALGVNAMLRK VAVAAASKPH VEIRQDGDQF YIKTSTTVRT TEINFKVGEG FEEETVDGRK CRSLPTWENE NKIHCTQTLL EGDGPKTYWT RELANDELIL TFGADDVVCT RIYVRE
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Attributs du produit
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Mouse Crabp1 Protein (raised in E. Coli) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Purification
    Two step purification of proteins expressed in bacterial culture:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Pureté
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Stérilité
    0.22 μm filtered
    niveau d'endotoxine
    Endotoxin has not been removed. Please contact us if you require endotoxin removal.
    Classe de qualité
    Crystallography grade
    Top Product
    Discover our top product CRABP1 Protéine
  • Indications d'application
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    Commentaires

    Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Conseil sur la manipulation
    Avoid repeated freeze-thaw cycles.
    Stock
    -80 °C
    Stockage commentaire
    Store at -80°C.
    Date de péremption
    Unlimited (if stored properly)
  • Antigène
    CRABP1 (Cellular Retinoic Acid Binding Protein 1 (CRABP1))
    Autre désignation
    Crabp1 (CRABP1 Produits)
    Synonymes
    CRABP Protein, CRABP-I Protein, CRABPI Protein, RBP5 Protein, CRABP1 Protein, cCRABP-I Protein, AI326249 Protein, Crabp-1 Protein, CrabpI Protein, Rbp-5 Protein, crabp1l Protein, crabpib Protein, crabp1 Protein, zgc:110497 Protein, cellular retinoic acid binding protein 1 Protein, cellular retinoic acid binding protein 1 S homeolog Protein, cellular retinoic acid binding protein I Protein, cellular retinoic acid binding protein 1b Protein, cellular retinoic acid binding protein 1a Protein, CRABP1 Protein, Crabp1 Protein, crabp1.S Protein, crabp1 Protein, crabp1b Protein, crabp1a Protein
    Sujet
    Cytosolic CRABPs may regulate the access of retinoic acid to the nuclear retinoic acid receptors.
    Poids moléculaire
    16.4 kDa Including tag.
    UniProt
    P62965
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