PRRX1 Protein (AA 1-245) (Strep Tag)

N° du produit ABIN3120819

Détail du produit

Antigène: PRRX1 (Paired Related Homeobox 1 (PRRX1))

Type de proteíne: Recombinant

Attributs du protein: AA 1-245

Origine: Souris

Source: Tobacco (Nicotiana tabacum)

Purification/Conjugué: Cette PRRX1 protéine est marqué à la Strep Tag.

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Séquence: MTSSYGHVLE RQPALGGRLD SPGNLDTLQA KKNFSVSHLL DLEEAGDMVA AQADESVGEA GRSLLESPGL TSGSDTPQQD NDQLNSEEKK KRKQRRNRTT FNSSQLQALE RVFERTHYPD AFVREDLARR VNLTEARVQV WFQNRRAKFR RNERAMLANK NASLLKSYSG DVTAVEQPIV PRPAPRPTDY LSWGTASPYS AMATYSATCA NNSPAQGINM ANSIANLRLK AKEYSLQRNQ VPTVN
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Attributs du produit: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

niveau d'endotoxine: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Commentaires:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: PRRX1 (Paired Related Homeobox 1 (PRRX1))

Autre désignation: Prrx1 (PRRX1 Produits)

Synonymes: AGOTC Protein, PHOX1 Protein, PMX1 Protein, PRX-1 Protein, PRX1 Protein, A230024N07Rik Protein, AA755424 Protein, AI385634 Protein, AI843499 Protein, K-2 Protein, Pmx Protein, Pmx1 Protein, Prx1 Protein, mHox Protein, Prx-1 Protein, gMHox Protein, homeobox Protein, pmx1 Protein, cb329 Protein, prrx1 Protein, zgc:64055 Protein, wu:fa09h05 Protein, wu:fb52c01 Protein, PRRX1 Protein, fa97b09 Protein, wu:fa97b09 Protein, zgc:101095 Protein, zgc:64199 Protein, paired related homeobox 1 Protein, paired related homeobox 1a Protein, paired related homeobox 1b Protein, PRRX1 Protein, Prrx1 Protein, prrx1a Protein, prrx1b Protein

Sujet: Paired mesoderm homeobox protein 1 (Homeobox protein K-2) (Muscle homeobox protein) (MHox) (Paired-related homeobox protein 1) (PRX-1),FUNCTION: Master transcription factor of stromal fibroblasts for myofibroblastic lineage progression. Orchestrates the functional drift of fibroblasts into myofibroblastic phenotype via TGF-beta signaling by remodeling a super-enhancer landscape. Through this function, plays an essential role in wound healing process (PubMed:35589735). Acts as a transcriptional regulator of muscle creatine kinase (MCK) and so has a role in the establishment of diverse mesodermal muscle types. The protein binds to an A/T-rich element in the muscle creatine enhancer (PubMed:1360403). May play a role in homeostasis and regeneration of bone, white adipose tissue and derm (PubMed:36456880). {ECO:0000269|PubMed:1360403, ECO:0000269|PubMed:35589735, ECO:0000269|PubMed:36456880}., FUNCTION: [Isoform 1]: Transcriptional activator, when transfected in fibroblastic or myoblastic cell lines. This activity may be masked by the C-terminal OAR domain. {ECO:0000269|PubMed:11373278}., FUNCTION: [Isoform 2]: Transcriptional repressor, when transfected in fibroblastic or myoblastic cell lines. {ECO:0000269|PubMed:11373278}.

Poids moléculaire: 27.3 kDa

UniProt: P63013