PIP5K1B Protein (AA 1-539) (Strep Tag)

N° du produit ABIN3120908

Détail du produit

Antigène: PIP5K1B (Phosphatidylinositol-4-Phosphate 5-Kinase, Type I, beta (PIP5K1B))

Type de proteíne: Recombinant

Attributs du protein: AA 1-539

Origine: Souris

Source: Tobacco (Nicotiana tabacum)

Purification/Conjugué: Cette PIP5K1B protéine est marqué à la Strep Tag.

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Séquence: MSSTAENGDA VPGKQNEEKT YKKTASSAIK GAIQLGIGYT VGNLTSKPER DVLMQDFYVV ESVFLPSEGS NLTPAHHYPD FRFKTYAPLA FRYFRELFGI KPDDYLYSIC SEPLIELSNP GASGSLFFLT SDDEFIIKTV QHKEAEFLQK LLPGYYMNLN QNPRTLLPKF YGLYCMQSGG INIRIVVMNN VLPRAMRMHL TYDLKGSTYK RRASRKEREK PNPTFKDLDF LQDMHEGLYF DTETYNALMK TLQRDCRVLE SFKIMDYSLL LGIHILDHSL KDKEEEPLQN VPDAKRPGMQ KVLYSTAMES IQGPGKSADG IIAENPDTMG GIPAKSHKGE KLLLFMGIID ILQSYRLMKK LEHSWKALVY DGDTVSVHRP SFYADRFLKF MNSRVFKKIQ ALKASPSKKR CNSIAALKAT SQEIVSSISQ EWKDEKRDLL TEGQSFSSLD EEALGSRHRP DLVPSTPSLF EAASLATTIS SSSLYVGEHY PHDRTTLYSN SKGLPSSSTF TLEEGTIYLT AEPNTLDLQD DASVLDVYL
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Attributs du produit: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

niveau d'endotoxine: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Commentaires:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: PIP5K1B (Phosphatidylinositol-4-Phosphate 5-Kinase, Type I, beta (PIP5K1B))

Autre désignation: Pip5k1b (PIP5K1B Produits)

Synonymes: MSS4 Protein, STM7 Protein, AI844522 Protein, Pip5k1a Protein, Pipk5a Protein, Pipk5b Protein, MGC80809 Protein, pip5k1b Protein, zgc:101046 Protein, wu:fj29b05 Protein, wu:fj93b05 Protein, zgc:92316 Protein, phosphatidylinositol-4-phosphate 5-kinase type 1 beta Protein, phosphatidylinositol-4-phosphate 5-kinase, type 1 beta Protein, phosphatidylinositol-4-phosphate 5-kinase, type I, beta L homeolog Protein, phosphatidylinositol-4-phosphate 5-kinase, type I, beta a Protein, phosphatidylinositol-4-phosphate 5-kinase, type I, beta Protein, phosphatidylinositol 4-phosphate 5-kinase type-1 beta Protein, phosphatidylinositol-4-phosphate 5-kinase, type I, beta b Protein, PIP5K1B Protein, Pip5k1b Protein, pip5k1b.L Protein, pip5k1ba Protein, pip5k1b Protein, LOC100472550 Protein, pip5k1bb Protein

Sujet: Phosphatidylinositol 4-phosphate 5-kinase type-1 beta (PIP5KI-beta) (PtdIns(4)P-5-kinase 1 beta) (EC 2.7.1.68) (68 kDa type I phosphatidylinositol 4-phosphate 5-kinase beta) (Phosphatidylinositol 4-phosphate 5-kinase type I alpha) (PIP5KIalpha) (Phosphatidylinositol 4-phosphate 5-kinase type I beta) (PIP5KIbeta),FUNCTION: Catalyzes the phosphorylation of phosphatidylinositol 4-phosphate (PtdIns(4)P/PI4P) to form phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2/PIP2), a lipid second messenger that regulates several cellular processes such as signal transduction, vesicle trafficking, actin cytoskeleton dynamics, cell adhesion, and cell motility (PubMed:8798574, PubMed:9367159, PubMed:9535851, PubMed:22942276). PtdIns(4,5)P2 can directly act as a second messenger or can be utilized as a precursor to generate other second messengers: inositol 1,4,5-trisphosphate (IP3), diacylglycerol (DAG) or phosphatidylinositol-3,4,5-trisphosphate (PtdIns(3,4,5)P3/PIP3) (By similarity). Mediates RAC1-dependent reorganization of actin filaments (PubMed:10679324). Contributes to the activation of phospholipase PLD2 (PubMed:11032811). Together with PIP5K1A, is required, after stimulation by G-protein coupled receptors, for the synthesis of IP3 that will induce stable platelet adhesion (PubMed:18772378). {ECO:0000250|UniProtKB:Q99755, ECO:0000269|PubMed:10679324, ECO:0000269|PubMed:11032811, ECO:0000269|PubMed:18772378, ECO:0000269|PubMed:22942276, ECO:0000269|PubMed:8798574, ECO:0000269|PubMed:9367159, ECO:0000269|PubMed:9535851}.

Poids moléculaire: 60.8 kDa

UniProt: P70181

Pathways: Signalisation PI3K-Akt, Inositol Metabolic Process, Cell-Cell Junction Organization