TANK Protein (AA 1-448) (Strep Tag)

N° du produit ABIN3120974

Détail du produit

Antigène: TANK (TRAF Family Member-Associated NFKB Activator (TANK))

Type de proteíne: Recombinant

Attributs du protein: AA 1-448

Origine: Souris

Source: Tobacco (Nicotiana tabacum)

Purification/Conjugué: Cette TANK protéine est marqué à la Strep Tag.

Application: SDS-PAGE (SDS), Western Blotting (WB), ELISA

Séquence: MSLKRHSLRR NACHLETRAG IPTILYSDAT GQRGMDKNIG EQLNRAYEAF RQACMDRDSA VRELQQKQTE NYEQRIREQQ EQLSFQQNLI DRLKSQLLLV DSSRDNSYGY VPLLEDSDRR KNNLTLDEPH DKVKLGTLRD KQSKVRRQEV SSGKESAKGL NIPLHHERDN IEKTFWDLKE EFHRICLLAK AQKDHLSKLN IPDIATDTQC SVPIQCTDKT EKQEALFKPQ AKDDINRGMS CVTAVTPRGL GRDEEDTSFE SLSKFNVKFP PMDNDSIFLH STPEAPSILA PATPETVCQD RFNMEVRDNP GNFVKTEETL FEIQGIDPIT SAIQNLKTTD KTNPSNLRAT CLPAGDHNVF YVNTFPLQDP PDAPFPSLDS PGKAVRGPQQ PFWKPFLNQD TDLVVPSDSD SELLKPLVCE FCQELFPPSI TSRGDFLRHL NTHFNGET
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Attributs du produit: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

niveau d'endotoxine: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Commentaires:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: TANK (TRAF Family Member-Associated NFKB Activator (TANK))

Autre désignation: Tank (TANK Produits)

Synonymes: I-TRAF Protein, ITRAF Protein, TRAF2 Protein, C86182 Protein, E430026L09Rik Protein, zgc:153048 Protein, i-traf Protein, tank Protein, traf2 Protein, TRAF family member associated NFKB activator Protein, TRAF family member-associated Nf-kappa B activator Protein, TRAF family member-associated NFKB activator Protein, TRAF family member associated NFKB activator L homeolog Protein, TANK Protein, Tank Protein, tank Protein, tank.L Protein

Sujet: TRAF family member-associated NF-kappa-B activator (TRAF-interacting protein) (I-TRAF),FUNCTION: Adapter protein involved in I-kappa-B-kinase (IKK) regulation which constitutively binds TBK1 and IKBKE playing a role in antiviral innate immunity. Acts as a regulator of TRAF function by maintaining them in a latent state. Blocks TRAF2 binding to LMP1 and inhibits LMP1-mediated NF-kappa-B activation. Negatively regulates NF-kappaB signaling and cell survival upon DNA damage. Plays a role as an adapter to assemble ZC3H12A, USP10 in a deubiquitination complex which plays a negative feedback response to attenuate NF-kappaB activation through the deubiquitination of IKBKG or TRAF6 in response to interleukin-1-beta (IL1B) stimulation or upon DNA damage. Promotes UBP10-induced deubiquitination of TRAF6 in response to DNA damage. May control negatively TRAF2-mediated NF-kappa-B activation signaled by CD40, TNFR1 and TNFR2. Essential for the efficient induction of IRF-dependent transcription following infection with Sendai virus. {ECO:0000250|UniProtKB:Q92844}.

Poids moléculaire: 50.9 kDa

UniProt: P70347

Pathways: Signalisation p53, Signalisation TLR, Activation of Innate immune Response