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RBP1 Protein (AA 2-135) (His tag)

Crystallography grade RBP1 Origine: Souris Hôte: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
N° du produit ABIN3121329
  • Antigène Voir toutes RBP1 Protéines
    RBP1 (Retinol Binding Protein 1, Cellular (RBP1))
    Type de proteíne
    Recombinant
    Attributs du protein
    AA 2-135
    Origine
    • 7
    • 3
    • 3
    • 2
    • 2
    Souris
    Source
    • 12
    • 2
    • 1
    • 1
    • 1
    Escherichia coli (E. coli)
    Purification/Conjugué
    Cette RBP1 protéine est marqué à la His tag.
    Application
    SDS-PAGE (SDS), Western Blotting (WB), ELISA, Crystallization (Crys)
    Séquence
    PVDFNGYWKM LSNENFEEYL RALDVNVALR KIANLLKPDK EIVQDGDHMI IRTLSTFRNY IMDFQVGKEF EEDLTGIDDR KCMTTVSWDG DKLQCVQKGE KEGRGWTQWI EGDELHLEMR AEGVICKQVF KKVH
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Attributs du produit
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Mouse Rbp1 Protein (raised in E. Coli) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Purification
    Two step purification of proteins expressed in bacterial culture:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Pureté
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Stérilité
    0.22 μm filtered
    niveau d'endotoxine
    Endotoxin has not been removed. Please contact us if you require endotoxin removal.
    Classe de qualité
    Crystallography grade
    Top Product
    Discover our top product RBP1 Protéine
  • Indications d'application
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    Commentaires

    Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Conseil sur la manipulation
    Avoid repeated freeze-thaw cycles.
    Stock
    -80 °C
    Stockage commentaire
    Store at -80°C.
    Date de péremption
    Unlimited (if stored properly)
  • Antigène
    RBP1 (Retinol Binding Protein 1, Cellular (RBP1))
    Autre désignation
    Rbp1 (RBP1 Produits)
    Synonymes
    RBBP-1 Protein, RBBP1 Protein, RBP-1 Protein, RBP1 Protein, CRABP-I Protein, CRBP Protein, CRBP1 Protein, CRBPI Protein, RBPC Protein, cb465 Protein, rbp1 Protein, wu:fb75e07 Protein, zgc:73335 Protein, CRABP1 Protein, Crbp Protein, Rbp-1 Protein, rbp1b Protein, sb:eu611 Protein, zgc:100825 Protein, AT-rich interaction domain 4A Protein, retinol binding protein 1 Protein, retinol binding protein 1a, cellular Protein, retinol binding protein 1 L homeolog Protein, retinol binding protein 1, cellular Protein, retinol binding protein 1b, cellular Protein, ARID4A Protein, RBP1 Protein, rbp5 Protein, rbp1.L Protein, rbp1 Protein, Rbp1 Protein
    Sujet
    Intracellular transport of retinol.
    Poids moléculaire
    16.7 kDa Including tag.
    UniProt
    Q00915
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