G2E3 Protein (AA 1-716) (Strep Tag)

N° du produit ABIN3122572

Détail du produit

Antigène: G2E3 (G2/M-Phase Specific E3 Ubiquitin Protein Ligase (G2E3))

Type de proteíne: Recombinant

Attributs du protein: AA 1-716

Origine: Souris

Source: Tobacco (Nicotiana tabacum)

Purification/Conjugué: Cette G2E3 protéine est marqué à la Strep Tag.

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Séquence: MNENKPDNSQ SLACVFCRKN DDCPNKYGEK KTYEKWNFSV HYYCLLMSSG IWQRGKEEEG VYGFLIEDIR KEVQRASKLK CTVCKKNGAS IGCVVPTCKR SYHLPCGLQK ECIFQFTDNF ASFCWKHRPV QAITSNKYSS SLPCTICLEF VEPIPTYNIL QSPCCKNAWF HRDCLQVQAI NAGVFFFRCT LCNNTDIFQK EMLRMGIHIP EKDASWELEE NAYQELLQSH DRCDIRRCHC KKGRDYNEPN SKWEVKRCQS CGSSGTHLAC SSLQSWEQNW ECLDCRRITY TSDFQKAPKH PLANSTNVTV TDCLLEESSS KLPRQSTVAQ HKELLRQGSK FRRDISTILI ELGFQIKKKT KTLYINKANV WRSALEQFQS QKFNPSCSID VVYVNGNEVG SQHLGSKQEF LSHLMHHLEN SSVFEGSLAK NLSLNSQAVK ENLYYEVGKM LAISLVHGGP SPGFFSETLF NCLAYGPENT LPTLDDVSDI DVAQIIIKID SATDLNILNS VISQHYNYLE VSGCLRLTTS LSDKFMLVKD ILFYHVINRV KAPFESFKQG LKTLGVLEKI QTYPEAFYKI LCHKPENLSA KNLSDLFTIH SVADVQTLRF WNSYLKAIED GKSATTMEDI LIFATGCSSV PPTGFKPSLS VECLHVDFPV ADKYRNHLVL PATNTYEEFQ ENMDFTIRDT LRLEKEERSH ILPRTLNVSS NEEMLI
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Attributs du produit: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

niveau d'endotoxine: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Commentaires:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: G2E3 (G2/M-Phase Specific E3 Ubiquitin Protein Ligase (G2E3))

Autre désignation: G2e3 (G2E3 Produits)

Synonymes: KIAA1333 Protein, PHF7B Protein, sb:cb726 Protein, si:busm1-234g15.1 Protein, si:dz234g15.1 Protein, wu:fe02a06 Protein, wu:fe24c12 Protein, 6030408C04Rik Protein, 9030416F18 Protein, AW046379 Protein, D930034K21Rik Protein, mKIAA1333 Protein, RGD1310263 Protein, G2/M-phase specific E3 ubiquitin protein ligase Protein, G2/M-phase specific E3 ubiquitin ligase Protein, G2E3 Protein, g2e3 Protein, G2e3 Protein

Sujet: G2/M phase-specific E3 ubiquitin-protein ligase (EC 2.3.2.26) (G2/M phase-specific HECT-type E3 ubiquitin transferase),FUNCTION: E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates (By similarity). Required for prevention of apoptotic death in early embryogenesis. {ECO:0000250|UniProtKB:Q7L622, ECO:0000269|PubMed:18511420}.

Poids moléculaire: 81.8 kDa

UniProt: Q5RJY2