HSPA4 Protein (AA 1-841) (His tag)
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- Antigène Voir toutes HSPA4 Protéines
- HSPA4 (Heat Shock 70kDa Protein 4 (HSPA4))
- Type de proteíne
- Recombinant
- Attributs du protein
- AA 1-841
- Origine
- Souris
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Source
- Cellules d'insectes
- Purification/Conjugué
- Cette HSPA4 protéine est marqué à la His tag.
- Application
- Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)
- Séquence
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MSVVGIDLGF QSCYVAVARA GGIETIANEY SDRCTPACVS FGPKNRSIGA AAKSQVISNA KNTVQGFKRF HGRAFSDPFV EAEKSNLAYD IVQLPTGLTG IKVTYMEEER NFTTEQVTAM LLSKLKETAE SVLKKPVVDC VVSVPSFYTD AERRSVMDAT QIAGLNCLRL MNETTAVALA YGIYKQDLPA LEEKPRNVVF VDMGHSAYQV SVCAFNKGKL KVLATAFDTT LGGRKFDEVL VNHFCEEFGK KYKLDIKSKI RALLRLSQEC EKLKKLMSAN ASDLPLSIEC FMNDIDVSGT MNRGKFLEMC DDLLARVEPP LRSVLEQSKL KKEDIYAVEI VGGATRIPAV KEKISKFFGK ELSTTLNADE AVTRGCALQC AILSPAFKVR EFSITDVVPY PISLRWNSPA EEGLSDCEVF PKNHAAPFSK VLTFYRKEPF TLEAYYSSPQ DLPYPDPAIA QFSVQKVTPQ SDGSSSKVKV KVRVNVHGIF SVSSAALVEV HKSEESEEPM ETDQNAKEEE KMQVDQEEPH TEEQQQQPQT PAENKAESEE METSQAGSKD KKTDQPPQAK KAKVKTSTVD LPIEHTLWQL DREMLALYTE NEGKMIMQDK LEKERNDAKN AVEEYVYEMR DKLSGEYEKF VSEDDRNTFT LKLEDTENWL YEDGEDQPKQ VYVDKLAELK SLGQPIKTRF QESEERPKLF EELGKQIQQY MKVISSFKNK EDQYEHLDAA DVTKVEKSTN EAMEWMNSKL NLQNKQSLTV DPVVKTKEIE AKIKELTSIC SPIISKPKPK VEPPKEEPKH AEQNGPVDGQ GDNPGSQAAE HGADTAVPSD GDKKLPEMDI D
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us. - Attributs du produit
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- Made in Germany - from design to production - by highly experienced protein experts.
- Mouse Hspa4 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).
When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- Purification
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Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
- In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- Pureté
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- Stérilité
- 0.22 μm filtered
- niveau d'endotoxine
- Protein is endotoxin free.
- Classe de qualité
- Crystallography grade
- Top Product
- Discover our top product HSPA4 Protéine
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- Indications d'application
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
- Commentaires
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Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
- Conseil sur la manipulation
- Avoid repeated freeze-thaw cycles.
- Stock
- -80 °C
- Stockage commentaire
- Store at -80°C.
- Date de péremption
- Unlimited (if stored properly)
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- Antigène
- HSPA4 (Heat Shock 70kDa Protein 4 (HSPA4))
- Autre désignation
- Hspa4 (HSPA4 Produits)
- Synonymes
- APG-2 Protein, HS24/P52 Protein, HSPH2 Protein, RY Protein, hsp70 Protein, hsp70RY Protein, Hsp110 Protein, Hsp70 Protein, irp94 Protein, 70kDa Protein, AI317151 Protein, Hsp70RY Protein, mKIAA4025 Protein, hspa4 Protein, wu:fi30e11 Protein, zgc:55743 Protein, zgc:77413 Protein, hs24/p52 Protein, hspa4-a Protein, osp94 Protein, pg-2 Protein, hspa4l Protein, wu:fc41d05 Protein, wu:fi59h02 Protein, wu:fj35c08 Protein, zgc:55506 Protein, heat shock protein family A (Hsp70) member 4 Protein, heat shock protein family A member 4 Protein, heat shock protein 4 Protein, heat shock protein 4b Protein, heat shock protein family A (Hsp70) member 4 S homeolog Protein, heat shock protein 4a Protein, HSPA4 Protein, Hspa4 Protein, hspa4b Protein, hspa4.S Protein, hspa4a Protein
- Poids moléculaire
- 95.1 kDa Including tag.
- UniProt
- Q61316
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