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Sonic Hedgehog Protein (SHH) (AA 199-437) (His tag)

Crystallography grade SHH Origine: Souris Hôte: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
N° du produit ABIN3123150
  • Antigène Voir toutes Sonic Hedgehog (SHH) Protéines
    Sonic Hedgehog (SHH)
    Type de proteíne
    Recombinant
    Attributs du protein
    AA 199-437
    Origine
    • 15
    • 12
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Souris
    Source
    • 18
    • 18
    • 6
    • 2
    • 1
    • 1
    • 1
    Escherichia coli (E. coli)
    Purification/Conjugué
    Cette Sonic Hedgehog protéine est marqué à la His tag.
    Application
    SDS-PAGE (SDS), Western Blotting (WB), ELISA, Crystallization (Crys)
    Séquence
    CFPGSATVHL EQGGTKLVKD LRPGDRVLAA DDQGRLLYSD FLTFLDRDEG AKKVFYVIET LEPRERLLLT AAHLLFVAPH NDSGPTPGPS ALFASRVRPG QRVYVVAERG GDRRLLPAAV HSVTLREEEA GAYAPLTAHG TILINRVLAS CYAVIEEHSW AHRAFAPFRL AHALLAALAP ARTDGGGGGS IPAAQSATEA RGAEPTAGIH WYSQLLYHIG TWLLDSETMH PLGMAVKSS
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Attributs du produit
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Mouse Shh Protein (raised in E. Coli) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Purification
    Two step purification of proteins expressed in bacterial culture:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Pureté
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Stérilité
    0.22 μm filtered
    niveau d'endotoxine
    Endotoxin has not been removed. Please contact us if you require endotoxin removal.
    Classe de qualité
    Crystallography grade
    Top Product
    Discover our top product SHH Protéine
  • Indications d'application
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    Commentaires

    Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Conseil sur la manipulation
    Avoid repeated freeze-thaw cycles.
    Stock
    -80 °C
    Stockage commentaire
    Store at -80°C.
    Date de péremption
    Unlimited (if stored properly)
  • Antigène
    Sonic Hedgehog (SHH)
    Autre désignation
    Shh (SHH Produits)
    Synonymes
    HHG1 Protein, HLP3 Protein, HPE3 Protein, MCOPCB5 Protein, SMMCI Protein, TPT Protein, TPTPS Protein, 9530036O11Rik Protein, Dsh Protein, Hhg1 Protein, Hx Protein, Hxl3 Protein, M100081 Protein, fc83d08 Protein, shh Protein, syu Protein, vhh-1 Protein, vhh1 Protein, wu:fc83d08 Protein, Xhh Protein, hedgehog Protein, xshh Protein, SHH Protein, twh Protein, twhh Protein, sonic hedgehog Protein, sonic hedgehog a Protein, sonic hedgehog L homeolog Protein, sonic hedgehog protein A Protein, sonic hedgehog b Protein, SHH Protein, Shh Protein, shha Protein, shh.L Protein, shh Protein, shhb Protein
    Sujet
    Intercellular signal essential for a variety of patterning events during development: signal produced by the notochord that induces ventral cell fate in the neural tube and somites, and the polarizing signal for patterning of the anterior-posterior axis of the developing limb bud. Displays both floor plate- and motor neuron-inducing activity. The threshold concentration of N-product required for motor neuron induction is 5-fold lower than that required for floor plate induction. Activates the transcription of target genes by interacting with its receptor PTCH1 to prevent normal inhibition by PTCH1 on the constitutive signaling activity of SMO. {ECO:0000269|PubMed:14687547, ECO:0000269|PubMed:7736596}.
    Poids moléculaire
    26.7 kDa Including tag.
    UniProt
    Q62226
    Pathways
    Signalisation Hedgehog, Dopaminergic Neurogenesis, Regulation of Muscle Cell Differentiation, Tube Formation, Skeletal Muscle Fiber Development
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