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CDC14A Protein (AA 1-603) (His tag)

Crystallography grade CDC14A Origine: Souris Hôte: Cellules d'insectes Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, Crys, SDS
N° du produit ABIN3123479
  • Antigène Voir toutes CDC14A Protéines
    CDC14A (CDC14 Cell Division Cycle 14 Homolog A (CDC14A))
    Type de proteíne
    Recombinant
    Attributs du protein
    AA 1-603
    Origine
    Souris
    Source
    • 2
    • 1
    Cellules d'insectes
    Purification/Conjugué
    Cette CDC14A protéine est marqué à la His tag.
    Application
    ELISA, Western Blotting (WB), Crystallization (Crys), SDS-PAGE (SDS)
    Séquence
    MAAESGELIG ACEFMKDRLY FATLRNRPKS TINIHYFSID EELVYENFYA DFGPLNLAMV YRYCCKLNKK LKSYSLSRKK IVHYTSFDQR KRANAAFLIG AYAVIYLKKT PEEAYRALLS GSNPPYLPFR DASFGNCTYN LTVLDCLQGI RKGLQHGFFD FETFDAEEYE HYERVENGDF NWIVPGKFLA FSGPHPKSKI ENGYPLHAPE AYFPYFKKNN VTTIVRLNKK IYEAKRFTDA GFEHYDLFFI DGSTPSDNIV RRFLNICENT EGAIAVHCKA GLGRTGTLIA CYVMKHYRFT HAEIIAWIRI CRPGSIIGPQ QHFLKEKQAS LWVQGDIFRS KLKNRPSSEG SITKIISTLD DMSIGANLSK LQSTERIGEN NFEDEDMEIK NNVTQGDKLR ALKSQRHPRS SPSCAFRSDD MKGHQRAMAQ TFRLSSSPQP TMSTMKTSKV CLSPSVTAKK ISRGSLSSGA NIRSFSINSR LASSLGNLNA GTEEPETKKT TSLTKAAFIA SPFTSFLNGS TQTPGRNYPE LNNNQYTRSS NSNSSSSSSG LGGNLNSSPV PQSAKPEEHT TILRPSFPGS LSSSSVRFLS RSIPSLQSEY VHY
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Attributs du produit
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Mouse Cdc14a Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Purification
    Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Pureté
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Stérilité
    0.22 μm filtered
    niveau d'endotoxine
    Protein is endotoxin free.
    Classe de qualité
    Crystallography grade
    Top Product
    Discover our top product CDC14A Protéine
  • Indications d'application
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    Commentaires

    Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Conseil sur la manipulation
    Avoid repeated freeze-thaw cycles.
    Stock
    -80 °C
    Stockage commentaire
    Store at -80°C.
    Date de péremption
    Unlimited (if stored properly)
  • Antigène
    CDC14A (CDC14 Cell Division Cycle 14 Homolog A (CDC14A))
    Autre désignation
    Cdc14a (CDC14A Produits)
    Synonymes
    cdc14 Protein, hCDC14 Protein, xcdc14a Protein, A830059A17Rik Protein, CDC14A2 Protein, CDC14a1 Protein, Cdc14 Protein, CDC14A Protein, zgc:63654 Protein, cell division cycle 14A Protein, Dual specificity protein phosphatase CDC14A Protein, dual specificity protein phosphatase CDC14A Protein, cell division cycle 14A L homeolog Protein, CDC14 cell division cycle 14A Protein, cell division cycle 14Aa Protein, CDC14A Protein, TVAG_168700 Protein, TVAG_479730 Protein, TVAG_362610 Protein, TVAG_490830 Protein, GL50803_9270 Protein, CMU_014610 Protein, cdc14a.L Protein, Cdc14a Protein, cdc14a Protein, cdc14aa Protein
    Sujet
    Dual-specificity phosphatase. Required for centrosome separation and productive cytokinesis during cell division. Dephosphorylates SIRT2 around early anaphase. May dephosphorylate the APC subunit FZR1/CDH1, thereby promoting APC-FZR1 dependent degradation of mitotic cyclins and subsequent exit from mitosis (By similarity). {ECO:0000250}.
    Poids moléculaire
    68.6 kDa Including tag.
    UniProt
    Q6GQT0
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