MBTD1 Protein (AA 1-631) (Strep Tag)

N° du produit ABIN3123618

Détail du produit

Antigène: MBTD1 (Mbt Domain Containing 1 (MBTD1))

Type de proteíne: Recombinant

Attributs du protein: AA 1-631

Origine: Souris

Source: Tobacco (Nicotiana tabacum)

Purification/Conjugué: Cette MBTD1 protéine est marqué à la Strep Tag.

Application: Western Blotting (WB), ELISA, SDS-PAGE (SDS)

Séquence: MFDGYDSCSE DTSSSSSSEE SEEEVAPLPS NLPIIKNNGQ VYTYPDGKSG MATCEMCGMV GVRDAFYSKT KRFCSVSCSR SYSSNSKKAS ILARLQGKPP TKKAKVLQKQ PLVAKLAAYA QYQATLQNQA KTKAGNSAIS VEGFSWGNYI NSNSFIAAPV ACFKHAPMGT CWGDISENVR IEVPNTDCSL PTKVFWIAGI IKLAGYNALL RYEGFENDSS LDFWCNICGS DIHPVGWCAA SGKPLVPPRT VQHKYTNWKA FLVKRLTGAK TLPPDFSQKV SESMQYPFKP CMRVEVVDKR HLCRTRVAVV ESVIGGRLRL VYEESEDRTD DFWCHMHSPL IHHIGWSRSI GHRFKRSDIT KKQDGHFDTP PHLFAKVKEV DQSGEWFKEG MKLEAIDPLN LSTICVATIR KVLADGFLMI GIDGSEAADG SDWFCYHATS PSIFPVGFCE INMIELTPPR GYTKLPFKWF DYLRETGSIA APVKLFNKDV PNHGFRVGMK LEAVDLMEPR LICVATVTRI IHRLLRIHFD GWEEEYDQWV DCESPDLYPV GWCQLTGYQL QPPASQSSRE SQSASSKQKK KAKSQQYKGH KKMTTSQLKE ELLDGEDYSF LHGASDQESN GSATVYIKQE P
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Attributs du produit: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

niveau d'endotoxine: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Commentaires:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: MBTD1 (Mbt Domain Containing 1 (MBTD1))

Autre désignation: Mbtd1 (MBTD1 Produits)

Synonymes: SA49P01 Protein, AA408199 Protein, AI194990 Protein, hemp Protein, mbt domain containing 1 Protein, mbt domain containing 1 L homeolog Protein, MBTD1 Protein, Mbtd1 Protein, mbtd1.L Protein

Sujet: MBT domain-containing protein 1,FUNCTION: Chromatin reader component of the NuA4 histone acetyltransferase complex, a multiprotein complex involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. The NuA4 complex plays a direct role in repair of DNA double-strand breaks (DSBs) by promoting homologous recombination (HR). MBTD1 specifically recognizes and binds monomethylated and dimethylated 'Lys-20' on histone H4 (H4K20me1 and H4K20me2, respectively). In the NuA4 complex, MBTD1 promotes recruitment of the complex to H4K20me marks by competing with TP53BP1 for binding to H4K20me. Following recruitment to H4K20me at DNA breaks, the NuA4 complex catalyzes acetylation of 'Lys-15' on histone H2A (H2AK15), blocking the ubiquitination mark required for TP53BP1 localization at DNA breaks, thereby promoting homologous recombination (HR). {ECO:0000250|UniProtKB:Q05BQ5}.

Poids moléculaire: 70.7 kDa

UniProt: Q6P5G3