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MARCH6 Protein (AA 164-283) (His tag)

Crystallography grade MARCH6 Origine: Souris Hôte: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, Crys, SDS
N° du produit ABIN3123904
  • Antigène Voir toutes MARCH6 Protéines
    MARCH6 (Membrane-Associated Ring Finger (C3HC4) 6, E3 Ubiquitin Protein Ligase (MARCH6))
    Type de proteíne
    Recombinant
    Attributs du protein
    AA 164-283
    Origine
    • 3
    • 1
    Souris
    Source
    • 2
    • 1
    • 1
    Escherichia coli (E. coli)
    Purification/Conjugué
    Cette MARCH6 protéine est marqué à la His tag.
    Application
    ELISA, Western Blotting (WB), Crystallization (Crys), SDS-PAGE (SDS)
    Séquence
    REQIVHGGAP IWLEHAAPPF NAAGHHQNEA PVGGNGAENP AADQPANPAG ENAVLGENPD AQDGQAEEEE EDNEEEDDAG VEDAADANNG AQDDMNWNAL EWDRAAEELT WERMLGLDGS
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Attributs du produit
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Mouse March6 Protein (raised in E. Coli) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Purification
    Two step purification of proteins expressed in bacterial culture:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Pureté
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Stérilité
    0.22 μm filtered
    niveau d'endotoxine
    Endotoxin has not been removed. Please contact us if you require endotoxin removal.
    Classe de qualité
    Crystallography grade
    Top Product
    Discover our top product MARCH6 Protéine
  • Indications d'application
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    Commentaires

    Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Conseil sur la manipulation
    Avoid repeated freeze-thaw cycles.
    Stock
    -80 °C
    Stockage commentaire
    Store at -80°C.
    Date de péremption
    Unlimited (if stored properly)
  • Antigène
    MARCH6 (Membrane-Associated Ring Finger (C3HC4) 6, E3 Ubiquitin Protein Ligase (MARCH6))
    Autre désignation
    March6 (MARCH6 Produits)
    Synonymes
    DOA10 Protein, MARCH-VI Protein, RNF176 Protein, TEB4 Protein, 3830408G03 Protein, F830029L24Rik Protein, mKIAA0597 Protein, RGD1310927 Protein, RGD1565757 Protein, MARCH6 Protein, im:7161807 Protein, wu:fa98a02 Protein, si:dkey-40c17.2 Protein, Mar-06 Protein, membrane associated ring-CH-type finger 6 Protein, membrane-associated ring finger (C3HC4) 6 Protein, membrane associated ring-CH-type finger 6 L homeolog Protein, membrane-associated ring finger (C3HC4) 6, E3 ubiquitin protein ligase Protein, MARCH6 Protein, March6 Protein, march6 Protein, march6.L Protein
    Sujet
    E3 ubiquitin-protein ligase that promotes ubiquitination of DIO2, leading to its degradation. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfer the ubiquitin to targeted substrates. May cooperate with UBE2G1 (By similarity). {ECO:0000250}.
    Poids moléculaire
    13.7 kDa Including tag.
    UniProt
    Q6ZQ89
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