NUCKS1 Protein (AA 1-234) (Strep Tag)

N° du produit ABIN3124436

Détail du produit

Antigène: NUCKS1 (Nuclear Casein Kinase and Cyclin-Dependent Kinase Substrate 1 (NUCKS1))

Type de proteíne: Recombinant

Attributs du protein: AA 1-234

Origine: Souris

Source: Tobacco (Nicotiana tabacum)

Purification/Conjugué: Cette NUCKS1 protéine est marqué à la Strep Tag.

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Séquence: MSRPVRNRKV VDYSQFQESD DADEDYGRDS GPPAKKIRSS PREAKNKRRS GKNSQEDSED SEEKDVKTKK DDSHSAEDSE DEKDDHKNVR QQRQAASKAA SKQREMLLED VGSEEEPEED DEAPFQEKDS GSDEDFLMED DDDSDYGSSK KKNKKMVKKS KPERKEKKMP KPRLKATVTP SPVKGKAKVG RPTASKKSKE KTPSPKEEDE EAESPPEKKS GDEGSEDEAS SGED
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Attributs du produit: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

niveau d'endotoxine: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Commentaires:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: NUCKS1 (Nuclear Casein Kinase and Cyclin-Dependent Kinase Substrate 1 (NUCKS1))

Autre désignation: Nucks1 (NUCKS1 Produits)

Synonymes: NUCKS Protein, jc7 Protein, MGC131005 Protein, fc07e02 Protein, nucks1 Protein, wu:fb16a10 Protein, wu:fb16h08 Protein, wu:fb36b01 Protein, wu:fb82a01 Protein, wu:fc07e02 Protein, wu:fe37f10 Protein, wu:gcd26e12 Protein, zgc:86878 Protein, Nucks Protein, 2700010L10Rik Protein, 8430423A01Rik Protein, AI647518 Protein, C78391 Protein, JC7 Protein, nucks Protein, fd12f12 Protein, wu:fd12f12 Protein, zgc:165430 Protein, nuclear casein kinase and cyclin dependent kinase substrate 1 Protein, nuclear casein kinase and cyclin-dependent kinase substrate 1 L homeolog Protein, nuclear casein kinase and cyclin-dependent kinase substrate 1a Protein, nuclear casein kinase and cyclin-dependent kinase substrate 1 Protein, nuclear casein kinase and cyclin-dependent kinase substrate 1b Protein, NUCKS1 Protein, nucks1.L Protein, nucks1a Protein, Nucks1 Protein, nucks1 Protein, nucks1b Protein

Sujet: Nuclear ubiquitous casein and cyclin-dependent kinase substrate 1 (JC7),FUNCTION: Chromatin-associated protein involved in DNA repair by promoting homologous recombination (HR). Binds double-stranded DNA (dsDNA) and secondary DNA structures, such as D-loop structures, but with less affinity than RAD51AP1. {ECO:0000250|UniProtKB:Q9H1E3}.

Poids moléculaire: 26.3 kDa

UniProt: Q80XU3