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CPEB2 Protein (AA 1-521) (His tag)

Crystallography grade CPEB2 Origine: Souris Hôte: Cellules d'insectes Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. Crys, ELISA, SDS, WB
N° du produit ABIN3124573
  • Antigène Voir toutes CPEB2 Protéines
    CPEB2 (Cytoplasmic Polyadenylation Element Binding Protein 2 (CPEB2))
    Type de proteíne
    Recombinant
    Attributs du protein
    AA 1-521
    Origine
    Souris
    Source
    • 1
    Cellules d'insectes
    Purification/Conjugué
    Cette CPEB2 protéine est marqué à la His tag.
    Application
    Crystallization (Crys), ELISA, SDS-PAGE (SDS), Western Blotting (WB)
    Séquence
    MNLPQQQPPA AAPQQPQSRR SPVSPQLQQQ HQAAAAAFLQ QRNSYNHHQP LLKQSPWSNH QNSGWGTASM SWGAMHGRDH RRSGNMGIPG TMNQISPLKK PFSGNVIAPP KFTRSTPSLT PKSWIEDNVF RTDNNSNTLL PLQVRSSLQL PAWGSDSLQD SWCTAAGTSR IDQDRSRMYD SLNMHSLENS LIDIMRAEHD PLKGRLSYPH PGTDNLLMLN GRSSLFPIDD SLLDDGHSDQ VGVLNSPTCY SAHQNGERIE RFSRKVFVGG LPPDIDEDEI TASFRRFGPL VVDWPHKAES KSYFPPKGYA FLLFQEESSV QALIDACIEE DGKLYLCVSS PTIKDKPVQI RPWNLSDSDF VMDGSQPLDP RKTIFVGGVP RPLRAVELAM IMDRLYGGVC YAGIDTDPEL KYPKGAGRVA FSNQQSYIAA ISARFVQLQH GDIDKRVEVK PYVLDDQMCD ECQGARCGGK FAPFFCANVT CLQYYCEFCW ANIHSRAGRE FHKPLVKEGA DRPRQIHFRW N
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Attributs du produit
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Mouse Cpeb2 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Purification
    Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Pureté
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Stérilité
    0.22 μm filtered
    niveau d'endotoxine
    Protein is endotoxin free.
    Classe de qualité
    Crystallography grade
    Top Product
    Discover our top product CPEB2 Protéine
  • Indications d'application
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    Commentaires

    Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Conseil sur la manipulation
    Avoid repeated freeze-thaw cycles.
    Stock
    -80 °C
    Stockage commentaire
    Store at -80°C.
    Date de péremption
    Unlimited (if stored properly)
  • Antigène
    CPEB2 (Cytoplasmic Polyadenylation Element Binding Protein 2 (CPEB2))
    Autre désignation
    Cpeb2 (CPEB2 Produits)
    Synonymes
    CPE-BP2 Protein, CPEB-2 Protein, hCPEB-2 Protein, A630055H10Rik Protein, Cpe-bp2 Protein, CPEB2 Protein, DKFZp469M211 Protein, cytoplasmic polyadenylation element binding protein 2 Protein, cytoplasmic polyadenylation element-binding protein 2 Protein, CPEB2 Protein, Cpeb2 Protein, cpeb2 Protein, LOC100351062 Protein
    Sujet
    May play a role in translational regulation of stored mRNAs in transcriptionally inactive haploid spermatids. Binds to poly(U) RNA oligomers.
    Poids moléculaire
    59.4 kDa Including tag.
    UniProt
    Q812E0
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