DCAF12 Protein (AA 1-453) (Strep Tag)

N° du produit ABIN3124745

Détail du produit

Antigène: DCAF12 (DDB1 and CUL4 Associated Factor 12 (DCAF12))

Type de proteíne: Recombinant

Attributs du protein: AA 1-453

Origine: Souris

Source: Tobacco (Nicotiana tabacum)

Purification/Conjugué: Cette DCAF12 protéine est marqué à la Strep Tag.

Application: ELISA, SDS-PAGE (SDS), Western Blotting (WB)

Séquence: MARKAVSRKR KASASPGAGS DAQGPQFGWD HSLHKRKRLP PVKRSLVYYL KNREVRLQNE TSYSRLLHGY AAQQLPSLLK EREFHLGTLN KVFASQWLNH RQVVCGTKCN TLFVVDVQTG QITKIPILKD REPGGVTQQG CGIHAIELNP SRTLLATGGD NPNSLAIYRL PTLDPVCVGD DGHKDWIFSI AWINDTMAVS GSRDGSMGLW EVTDDVLTKS DARHNVSPVP VYAHITHKAL KDIPKEDTNP DNCKVRALAF NNKNKELGAV SLDGYFHLWK AENTLSKLLS TKLPYCRENV CLAYGSEWSV YAVGSQAHVS FLDPRQPSYN VKSVCSRERG SGIRSVSFYE HIITVGTGQG SLLFYDIRAQ RFLEERLSAC YGSKPRLAGE NLKLTTGRGW LNHDETWRNY FSDIDFFPNA VYTHCYDSSG TKLFVAGGPL PSGLHGNYAG LWS
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Attributs du produit: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

niveau d'endotoxine: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Commentaires:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: DCAF12 (DDB1 and CUL4 Associated Factor 12 (DCAF12))

Autre désignation: Dcaf12 (DCAF12 Produits)

Synonymes: CT102 Protein, KIAA1892 Protein, TCC52 Protein, WDR40A Protein, 1500001L20Rik Protein, 5830424K06Rik Protein, AA420338 Protein, AI851081 Protein, Wdr40a Protein, wdr40a Protein, wu:fb93d04 Protein, zgc:154031 Protein, wdr40b Protein, dcaf12 Protein, wdr40a-a Protein, DDB1 and CUL4 associated factor 12 Protein, DDB1 and CUL4 associated factor 12 L homeolog Protein, DCAF12 Protein, Dcaf12 Protein, dcaf12 Protein, dcaf12.L Protein

Sujet: DDB1- and CUL4-associated factor 12 (WD repeat-containing protein 40A),FUNCTION: Substrate-recognition component of a DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complex of the DesCEND (destruction via C-end degrons) pathway, which recognizes a C-degron located at the extreme C terminus of target proteins, leading to their ubiquitination and degradation. The C-degron recognized by the DesCEND pathway is usually a motif of less than ten residues and can be present in full-length proteins, truncated proteins or proteolytically cleaved forms (PubMed:34065512). The DCX(DCAF12) complex specifically recognizes proteins with a diglutamate (Glu-Glu) at the C-terminus, such as MAGEA3, MAGEA6 and CCT5, leading to their ubiquitination and degradation. Ubiquitination of MAGEA3, MAGEA6 by DCX(DCAF12) complex is required for starvation-induced autophagy (By similarity). Also directly recognizes the C-terminal glutamate-leucine (Glu-Leu) degron as an alternative degron in proteins such as MOV10, leading to their ubiquitination and degradation. Controls the protein level of MOV10 during spermatogenesis and in T cells, especially after their activation (PubMed:34065512). {ECO:0000250|UniProtKB:Q5T6F0, ECO:0000269|PubMed:34065512}.

Poids moléculaire: 50.5 kDa

UniProt: Q8BGZ3