EIF2A Protein (AA 2-581) (His tag)

N° du produit ABIN3124938

Détail du produit

Antigène: EIF2A (Eukaryotic Translation Initiation Factor 2A, 65kDa (EIF2A))

Type de proteíne: Recombinant

Attributs du protein: AA 2-581

Origine: Souris

Source: Cellules d'insectes

Purification/Conjugué: Cette EIF2A protéine est marqué à la His tag.

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS), Crystallization (Crys)

Séquence: APSTPLLTVR GSEGLYMVNG PPHFTESTVL PRESGRNCKV YTFSKDGTLF AWSNGEKVNI INVANKGLLH SFDLPKAVCL EFSPNNTVLA TWQPYTTSKD GTAGTPNLQL YDMKTGACLK SFIQKKMQNW CPSWSDDEII CARNVNNEVH FFENNNFNTI ANKLHLQKVN DFNLSPGTQP YKVAVYVPGS KGAPSFVRLY QYPNFAGPQA ALANKSFFKA DKVTMLWNKK ATAVLVIAST EVDKTGASYY GEQTLHYIAT NGESAVVQLP KNGPIYDVVW NSSSTEFCAV YGFMPAKATV FNLKCDPVFD FGTGPRNAAF YSPHGHILVL AGFGNLRGQM EVWDVKNYKL ISKPVASDST YFAWCPDGEH ILTATCAPRL RVNNGYKIWH YTGSLLHKYD VPSNGELWQV SWQPFLDGIF PAKTIKYQAV PSEVPSEEPK VATAYRPPAL RNKPVTNSKL HEEEPPQNMK PHPGSDKPLS KTALKNQRKH EAKKAAKQEA RSDAAPTPVP QSAPRNTVTQ SASGDPEVDK KIKNLKKKLK AIEQLKEQAA AGKQLEKNQL EKIQKETALL QELEDLELGV
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Attributs du produit:

• Made in Germany - from design to production - by highly experienced protein experts.
• Mouse Eif2a Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Protein is endotoxin free.

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Commentaires:

Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: EIF2A (Eukaryotic Translation Initiation Factor 2A, 65kDa (EIF2A))

Autre désignation: Eif2a (EIF2A Produits)

Synonymes: ATEIF2-A2 Protein, EIF2-A2 Protein, eukaryotic translation initiation factor 2 alpha subunit Protein, EIF-2A Protein, MST089 Protein, MSTP004 Protein, MSTP089 Protein, D030048D22 Protein, D3Ertd194e Protein, im:7145971 Protein, zgc:110460 Protein, DDBDRAFT_0185936 Protein, DDBDRAFT_0234177 Protein, DDB_0185936 Protein, DDB_0234177 Protein, Eif2a Protein, eif2a Protein, eif2alpha Protein, EIF2A Protein, CG9946 Protein, Dmel\\CG9946 Protein, Eif2alpha Protein, IF2A_DROME Protein, M(1)14C Protein, M(1)19-153 Protein, M(1)8e3-10 Protein, deIF2alpha Protein, eIF-2 Protein, eIF-2 a Protein, eIF-2 alpha Protein, eIF2 alpha Protein, eIF2-alpha Protein, eIF2a Protein, eIF2alpha Protein, eiF-2alpha Protein, elF2alpha Protein, l(1)14Cf Protein, l(1)19-153 Protein, eukaryotic translation initiation factor 2 alpha subunit Protein, eukaryotic translation initiation factor 2A Protein, eukaryotic translation initiation factor 2A, 65kDa Protein, eukaryotic translation initiation factor 2A S homeolog Protein, eukaryotic translation initiation factor Protein, eukaryotic translation Initiation Factor 2alpha Protein, EIF2 ALPHA Protein, EIF2A Protein, Eif2a Protein, eif2a Protein, eIF2a Protein, eif2a.S Protein, eif2A Protein, eIF-2alpha Protein

Sujet: Functions in the early steps of protein synthesis of a small number of specific mRNAs. Acts by directing the binding of methionyl-tRNAi to 40S ribosomal subunits. In contrast to the eIF-2 complex, it binds methionyl-tRNAi to 40 S subunits in a codon-dependent manner, whereas the eIF-2 complex binds methionyl-tRNAi to 40 S subunits in a GTP-dependent manner. May act by impiging the expression of specific proteins (By similarity). {ECO:0000250}.

Poids moléculaire: 65.2 kDa Including tag.

UniProt: Q8BJW6

Pathways: Ribonucleoprotein Complex Subunit Organization, ER-Nucleus Signaling, Hepatitis C, Methionine Biosynthetic Process, Ribosome Assembly