SRP68 Protein (AA 1-625) (Strep Tag)

N° du produit ABIN3125035

Détail du produit

Antigène: SRP68 (Signal Recognition Particle 68kDa (SRP68))

Type de proteíne: Recombinant

Attributs du protein: AA 1-625

Origine: Souris

Source: Tobacco (Nicotiana tabacum)

Purification/Conjugué: Cette SRP68 protéine est marqué à la Strep Tag.

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Séquence: MAAEKQIPGG GSGGGGSGSG GGGGGSGGGR SAGGDENKEN ERPSAGSKAN KEFGDSLSLE ILQIIKESQQ QHGLRHGDFQ RYRGYCSRRQ RRLRKTLNFK MGNRHKFTGK KVTEELLTDN RYLLLVLMDA ERAWSYAMQL KQEANTEPRK RFHLLSRLRK AVKHAEELER LCESNRVDAK TKLEAQAYTA YLSGMLRFEH QEWKSAIEAF NKCKTIYEKL ASAFTEEQAV LYNQRVEEIS PNIRYCAYNI GDQSAINELM QMRLRSGGTE GLLAEKLEAL ITQTRAKQAA TMSEVEWRGR TVPVKIDKVR IFLLGLADNE AAIVQAESEE TKERLFESML SECRDALQAV REELKPDQKQ RDYALDGESG KVSNLQYLHS YLTYIKLSTA IRRNENMAKG LHRALLQQQP EDDSKRSPRP QDLIRLYDII LQNLVELLQL PGLEEDRTFQ KEISLKTLVF KAYRCFFIAQ SYVLVKKWSE ALVLYDRVLK YANEVSSHGG ASKNSLKDLP DVQELITQVR SEKCSLQAAA ILDANDSHQT DTSSQVKDNT PLVERFESFC LDPSLVTKQA NLVHFPPGFQ PIPCKPLFFD LALNHVAFPP LEDKLEQKTK SGLTGYIKGI FGFRS
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Attributs du produit: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

niveau d'endotoxine: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Commentaires:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: SRP68 (Signal Recognition Particle 68kDa (SRP68))

Autre désignation: Srp68 (SRP68 Produits)

Synonymes: 2610024I03Rik Protein, CG5064 Protein, Dmel\\CG5064 Protein, zgc:92573 Protein, Signal recognition particle 68kDa Protein, signal recognition particle 68 Protein, Signal recognition particle protein 68 Protein, signal recognition particle 68kDa L homeolog Protein, GL50803_8916 Protein, SRP68 Protein, Srp68 Protein, srp68 Protein, srp68.L Protein

Sujet: Signal recognition particle subunit SRP68 (SRP68) (Signal recognition particle 68 kDa protein),FUNCTION: Component of the signal recognition particle (SRP) complex, a ribonucleoprotein complex that mediates the cotranslational targeting of secretory and membrane proteins to the endoplasmic reticulum (ER) (By similarity). The SRP complex interacts with the signal sequence in nascent secretory and membrane proteins and directs them to the membrane of the ER (By similarity). The SRP complex targets the ribosome-nascent chain complex to the SRP receptor (SR), which is anchored in the ER, where SR compaction and GTPase rearrangement drive cotranslational protein translocation into the ER (By similarity). Binds the signal recognition particle RNA (7SL RNA), SRP72 binds to this complex subsequently (By similarity). The SRP complex possibly participates in the elongation arrest function (By similarity). {ECO:0000250|UniProtKB:P38687, ECO:0000250|UniProtKB:Q9UHB9}.

Poids moléculaire: 70.6 kDa

UniProt: Q8BMA6