Tel:
+49 (0)241 95 163 153
Fax:
+49 (0)241 95 163 155
E-Mail:
orders@anticorps-enligne.fr

NOB1 Protein (AA 2-403) (His tag)

Crystallography grade NOB1 Origine: Souris Hôte: Cellules d'insectes Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, SDS, WB, Crys
N° du produit ABIN3125348
  • Antigène Voir toutes NOB1 Protéines
    NOB1 (RNA-Binding Protein NOB1 (NOB1))
    Type de proteíne
    Recombinant
    Attributs du protein
    AA 2-403
    Origine
    • 4
    • 1
    • 1
    • 1
    Souris
    Source
    • 3
    • 1
    • 1
    • 1
    • 1
    Cellules d'insectes
    Purification/Conjugué
    Cette NOB1 protéine est marqué à la His tag.
    Application
    ELISA, SDS-PAGE (SDS), Western Blotting (WB), Crystallization (Crys)
    Séquence
    APVEHVVADA GAFLRDAPLQ DIGKNIYTIR EVVREIRDKA TRRRLAVLPY QLRFKEPLPE YVRLVTEFSK KTGDYPSLSA TDIQVLALTY QLEAEFVGVS HLKKEPEKAK VSSSIQHPET ALHISGFHLP SKSKPLQEAV DRGHAADGPE NLEFSSFMFW RTPLPNIDRE LQELLIDGRE EEEEEEECED SDDDGGGWIT PSNIKQIQQE LEQCDTPEDV QVGCVTTDFA MQNVLLQMGL HVLAVNGMLV REARSYILRC HGCFKTTSDM NRVFCGHCGN KTLKKVSVTI NDDGTLHMHF SRNPKVLNPR GLRYSLPTPK GGKYAINPHL TEDQRFPQLR LSQKARQKTD VFAPDYIAGV SPFAENDISS RSAILQVRDG MLGAGRRRLN PNASRKKFVK KR
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Attributs du produit
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Mouse Nob1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Purification
    Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Pureté
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Stérilité
    0.22 μm filtered
    niveau d'endotoxine
    Protein is endotoxin free.
    Classe de qualité
    Crystallography grade
    Top Product
    Discover our top product NOB1 Protéine
  • Indications d'application
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    Commentaires

    Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Conseil sur la manipulation
    Avoid repeated freeze-thaw cycles.
    Stock
    -80 °C
    Stockage commentaire
    Store at -80°C.
    Date de péremption
    Unlimited (if stored properly)
  • Antigène
    NOB1 (RNA-Binding Protein NOB1 (NOB1))
    Autre désignation
    Nob1 (NOB1 Produits)
    Synonymes
    MEE6.26 Protein, MEE6_26 Protein, Nob1p Protein, fc27e05 Protein, si:ch73-167i17.3 Protein, wu:fc27e05 Protein, ART-4 Protein, MST158 Protein, NOB1P Protein, PSMD8BP1 Protein, 1700021I09Rik Protein, Psmd8bp1 Protein, NIN1/PSMD8 binding protein 1 homolog Protein, RNA-binding NOB1-like protein Protein, RNA-binding protein nob1 Protein, RNA-binding protein NOB1 Protein, NIN1/RPN12 binding protein 1 homolog S homeolog Protein, NIN1/RPN12 binding protein 1 homolog (S. cerevisiae) Protein, NIN1/RPN12 binding protein 1 homolog Protein, NOB1 Protein, AT5G41190 Protein, EDI_130960 Protein, CpipJ_CPIJ012739 Protein, BDBG_01281 Protein, PAAG_04204 Protein, PITG_13300 Protein, nob1.S Protein, nob1 Protein, Nob1 Protein
    Sujet
    May play a role in mRNA degradation. {ECO:0000250}.
    Poids moléculaire
    46.3 kDa Including tag.
    UniProt
    Q8BW10
    Pathways
    Protein targeting to Nucleus
Vous êtes ici:
Support technique