TRIM35 Protein (AA 1-501) (Strep Tag)

N° du produit ABIN3125531

Détail du produit

Antigène: TRIM35 (Tripartite Motif Containing 35 (TRIM35))

Type de proteíne: Recombinant

Attributs du protein: AA 1-501

Origine: Souris

Source: Tobacco (Nicotiana tabacum)

Purification/Conjugué: Cette TRIM35 protéine est marqué à la Strep Tag.

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Séquence: MEPGPSVSPG PSRSFKEELL CAVCYDPFRD AVTLRCGHNF CRRCVSGCWE VQTTPSCPVC KERAVPGELR TNHTLNNLVE TLLREEAEGA RWTGRRSPRP CRAHRAPLTL FCLEDKELLC CACQADARHQ EHRVQPIKDT AQDFRAKCKN MEHVLREKAK AFWALRRTYE AIAKHNEVQT TWLEGRIRDE FDKLRDFLRV EEQATLDAMK EESRKKHLQA EEKMKQLAEQ TEALAREIER LQMEMKEDDM TFLMKHKSRK RRLFCTVEPA PLQPGLLMDA CKYLESLQYR VWKKMLGSVE SVPFSLDPNT AAGWLKVADD LTSVINHGYR VQVENPERFS SAPCLLGSQV FSKGSHSWEV DVGGLPTWRV GVVRVQAHAQ AQAQADVGGE GHSHSCYHDT RSGFWYLCRT QGVDGDHCMT SDTATAPLVQ AMPRRLRVEL ECEEGELSFY DSERHCHLYT FHAHFGEVRP YFYLGASRGD GPPEPLRICH LRVSIKEELD I
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Attributs du produit: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

niveau d'endotoxine: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Commentaires:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: TRIM35 (Tripartite Motif Containing 35 (TRIM35))

Autre désignation: Trim35 (TRIM35 Produits)

Synonymes: trim35 Protein, wu:fi37h05 Protein, zgc:63737 Protein, TRIM35 Protein, DKFZp468B2113 Protein, HLS5 Protein, MAIR Protein, 0710005M05Rik Protein, A430106H13Rik Protein, AW046487 Protein, Mair Protein, NC8 Protein, mKIAA1098 Protein, tripartite motif containing 35 Protein, tripartite motif containing 35-27 Protein, tripartite motif-containing 35 Protein, TRIM35 Protein, trim35-27 Protein, Trim35 Protein

Sujet: E3 ubiquitin-protein ligase TRIM35 (EC 2.3.2.27) (Hemopoietic lineage switch protein 5) (Macrophage-derived apoptosis-inducing RBCC protein) (Protein MAIR) (Protein Nc8),FUNCTION: E3 ubiquitin-protein ligase that participates in multiple biological processes including cell death, glucose metabolism, and in particular, the innate immune response (By similarity) (PubMed:32562145). Mediates 'Lys-63'-linked polyubiquitination of TRAF3 thereby promoting type I interferon production via RIG-I signaling pathway. Can also catalyze 'Lys-48'-linked polyubiquitination and proteasomal degradation of viral proteins such as influenza virus PB2. Acts as a negative feedback regulator of TLR7- and TLR9-triggered signaling. Mechanistically, promotes the 'Lys-48'-linked ubiquitination of IRF7 and induces its degradation via a proteasome-dependent pathway. Reduces FGFR1-dependent tyrosine phosphorylation of PKM, inhibiting PKM-dependent lactate production, glucose metabolism, and cell growth (By similarity). {ECO:0000250|UniProtKB:Q9UPQ4, ECO:0000269|PubMed:32562145}.

Poids moléculaire: 57.3 kDa

UniProt: Q8C006