EIF2B5 Protein (AA 1-717) (Strep Tag)

N° du produit ABIN3126047

Détail du produit

Antigène: EIF2B5 (Eukaryotic Translation Initiation Factor 2B, Subunit 5 Epsilon, 82kDa (EIF2B5))

Type de proteíne: Recombinant

Attributs du protein: AA 1-717

Origine: Souris

Source: Tobacco (Nicotiana tabacum)

Purification/Conjugué: Cette EIF2B5 protéine est marqué à la Strep Tag.

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Séquence: MAATAAVPGA AAGRASKRGG GGSGGGGTQG AEEEPPPPLQ AVLVADSFNR RFFPISKDQP RVLLPLANVA LIDYTLEFLT ATGVQETFVF CCWKAAQIKE HLQKSKWCHP TSPNVVRIIT SELYRSLGDV LRDVDAKALV RSDFLLIYGD VISNINICRA LEEHRLRRKL EKNVSVMTMV FKESSPSHPT RCHEDNVVMA VDSATNRVLH FQKTQGLRRF SFPLSLFQGS GDGVEIRYDL LDCHISICSP QVAQLFTDNF DYQTRDDFVR GILMNEEVLG NQIHLHVTTR EYGARVSNLH MYSAVCADVI RRWVYPLTPE VNFTDSTTQS YTHSRHNIYR GPEVSLGHGS VLEENVLLGA GTVIGSNCSI TNSVIGPNCH IGDNVVLDQA YLWQGVRVAA GAQIHQSLLC DRAEVKERVK LKPYCVLTSQ VVVGPDITLP EGSVISLHPP DAEEDEDDGQ FSDDSGADQE KEKVKLKGYN PAEVGLEGQG YLWKAEGVNS KEDEELRQSL WGLMIKTEEE SETESEGSVD PEELDSRAGS PQLDDIRVFQ NEVLGTLQRG REENISCENL VLEINSLKHA YNISLKEVMQ VLTLVVLEFP LQQVDGLLDP NRYCALLLPL LKAWSPVLRN YIKRAADHLE ALAAIEDFFL EHETLVTSMA KVLMAFYQLE ILAEETILSW FSQRDTTDEG QQLRKNQQLQ RFIQWLREAE EESSEDD
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Attributs du produit: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

niveau d'endotoxine: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Commentaires:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: EIF2B5 (Eukaryotic Translation Initiation Factor 2B, Subunit 5 Epsilon, 82kDa (EIF2B5))

Autre désignation: Eif2b5 (EIF2B5 Produits)

Synonymes: EIF2B5 Protein, F19I3.20 Protein, F19I3_20 Protein, F14P3.8 Protein, F14P3_8 Protein, AO090003000364 Protein, C81315 Protein, CACH Protein, CLE Protein, EIF-2B Protein, EIF2Bepsilon Protein, LVWM Protein, eukaryotic translation initiation factor 2B subunit epsilon Protein, eukaryotic translation initiation factor 2B, subunit 5 epsilon, 82kDa Protein, Trimeric LpxA-like enzyme Protein, translation initiation factor eif-2b epsilon subunit Protein, translation initiation factor eIF-2B epsilon subunit Protein, Translation initiation factor eIF-2B epsilon subunit, putative Protein, eukaryotic translation initiation factor 2B, subunit 5 epsilon Protein, EIF2B5 Protein, AT2G34970 Protein, AT3G02270 Protein, AOR_1_624154 Protein, CTRG_05484 Protein, PAAG_05066 Protein, CGB_A5380C Protein, TERG_01115 Protein, Eif2b5 Protein

Sujet: Translation initiation factor eIF2B subunit epsilon (eIF2B GDP-GTP exchange factor subunit epsilon),FUNCTION: Acts as a component of the translation initiation factor 2B (eIF2B) complex, which catalyzes the exchange of GDP for GTP on eukaryotic initiation factor 2 (eIF2) gamma subunit. Its guanine nucleotide exchange factor activity is repressed when bound to eIF2 complex phosphorylated on the alpha subunit, thereby limiting the amount of methionyl-initiator methionine tRNA available to the ribosome and consequently global translation is repressed. {ECO:0000250|UniProtKB:Q13144}.

Poids moléculaire: 80.1 kDa

UniProt: Q8CHW4