Kringle Containing Transmembrane Protein 2 (KREMEN2) (AA 25-461) protein (rho-1D4 tag)

Détails pour le produit réf. ABIN3126289
Nom de la protéine
  • krm2
  • KREMEN2
  • MGC147633
  • KRM2
  • 2900054E04Rik
  • Krm2
  • kringle containing transmembrane protein 2 S homeolog
  • kringle containing transmembrane protein 2
  • kremen2.S
  • KREMEN2
  • kremen2
  • Kremen2
Attributs du protein
AA 25-461
1
1
1
1
1
1
Origine
Souris
5
1
Source
Cellules d'insectes
3
2
1
Type de proteíne
Recombinant
Purification/Conjugué
Cette KREMEN2 protéine est marqué à la rho-1D4 tag.
Application
Crystallization (Crys), ELISA, SDS-PAGE (SDS), Western Blotting (WB)
Options
Séquence GSLHSPGLSE CFQVNGADYR GHQNYTGPRG AGRPCLFWDQ TQQHSYSSAS DPQGRWGLGA HNFCRNPDGD VQPWCYVAET EEGIYWRYCD IPTCHMPGYL GCFVDSGAPP ALSGPSGTST KLTVQVCLRF CRMKGYQLAG VEAGYACFCG SESDLARGRP APATDCDQIC FGHPGQLCGG DGRLGIYEVS VGSCQGNWSA PQGVIYSPDF PDEYGPDRNC SWVLGQLGAV LELTFRLFEL ADSRDRLELR DVSSGNLLRA FDGAHPPPPG PLRLRTAALL LTFRSDARGH AQGFALTYRG LQDTVEGRAS PEDSTESLAG DPDGANASCS PKPGAAQASI GARVFSTVTA FSVLLLLLLS LLRLLRRRSC LLAPGKGSLA MGPSRGPGRS WAVWYRRPRG VALPCPPGDS QAEGPAAGYR PLSASSQSSL RSLVSAL
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
Attributs du produit
  • Made in Germany - from design to production - by highly experienced protein experts.
  • Mouse Kremen2 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
  • State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification Three step purification of membrane proteins expressed in baculovirus infected SF9 insect cells:
  1. Membrane proteins are fractioned by ultracentrifugation and subsequently solubilized with different detergents (detergent screen). Samples are analyzed by Western blot.
  2. The best performing detergent is used for solubilization and the proteins are purified via their rho1D4 tag via two rho1D4 antibody columns: one DTT resistant, the other one not. Eluate fractions are analyzed by Western blot.
  3. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatograph. Eluate fractions are analyzed by SDS-PAGE and Western blot.
Pureté >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
Stérilité 0.22 μm filtered
niveau d'endotoxine Protein is endotoxin-free.
Classe de qualité Crystallography grade
Nom de la protéine
Sujet Receptor for Dickkopf protein. Cooperates with Dickkopf to block Wnt/beta-catenin signaling. Forms a ternary complex with Dkk1 and LRP6 and induces rapid endocytosis and removal of the Wnt receptor LRP6 from the plasma membrane. {ECO:0000269|PubMed:12050670}.
Poids moléculaire 47.8 kDa Including tag.
UniProt Q8K1S7
Pathways Signalisation WNT
Indications d'application In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
Commentaires

Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions For Research Use only
Format Liquid
Buffer 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
Conseil sur la manipulation Avoid repeated freeze-thaw cycles.
Stock -80 °C
Stockage commentaire Store at -80°C.
Date de péremption Unlimited (if stored properly)
Images (Fournisseur)
Image no. 1 for Kringle Containing Transmembrane Protein 2 (KREMEN2) (AA 25-461) protein (rho-1D4 tag) (ABIN3126289) „Crystallography Grade“ protein due to multi-step, protein-specific purification process
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