PPHLN1 Protein (AA 1-381) (Strep Tag)

N° du produit ABIN3126333

Détail du produit

Antigène: PPHLN1 (Periphilin 1 (PPHLN1))

Type de proteíne: Recombinant

Attributs du protein: AA 1-381

Origine: Souris

Source: Tobacco (Nicotiana tabacum)

Purification/Conjugué: Cette PPHLN1 protéine est marqué à la Strep Tag.

Application: Western Blotting (WB), SDS-PAGE (SDS), ELISA

Séquence: MWSEGRYDYD RLPRERVPPR SHPSDGYHRV VNVVPKRPPL LDKRPPLLDK RPPLLARPDE GGYSRYYSHV DCRVCDEGRS FSHDRRSGPS HSGDESGYRW LRDDHSTSRQ PDYRDMRDGF RRKSFYSSHY SRDRSPHKRD APFFRESPVG RKDSPHSRSG SSVSSRSYSP ERSRTHSFHQ SQHRKSSRVG ASYKRQNEAI RGRGKERSIQ SVKTSRDASP SSSSAVASSK ALDKPSRLTE KELAEAESKW ANETLEKSDE SNLAEMNEFE AGSTAPLFID QTEEPESNTV DGTELYEDSQ LSNRSKAIAS KTKEIEQVYR QDCETFGMVV KMLIEKDPSL EKSVQFALRQ NLHEIGERCV EELKRFITEY DNSARDFGDP F
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Attributs du produit: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

niveau d'endotoxine: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Commentaires:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: PPHLN1 (Periphilin 1 (PPHLN1))

Autre désignation: Pphln1 (PPHLN1 Produits)

Synonymes: MGC75908 Protein, MGC81987 Protein, wu:fb83c09 Protein, zgc:158246 Protein, si:ch211-155a11.2 Protein, HSPC232 Protein, 1110063K05Rik Protein, 1600022A19Rik Protein, CR Protein, HSPC206 Protein, periphilin 1 Protein, periphilin 1 S homeolog Protein, PPHLN1 Protein, pphln1 Protein, pphln1.S Protein, Pphln1 Protein

Sujet: Periphilin-1,FUNCTION: Component of the HUSH complex, a multiprotein complex that mediates epigenetic repression. The HUSH complex is recruited to genomic loci rich in H3K9me3 and is probably required to maintain transcriptional silencing by promoting recruitment of SETDB1, a histone methyltransferase that mediates further deposition of H3K9me3. In the HUSH complex, contributes to the maintenance of the complex at chromatin. Acts as a transcriptional corepressor and regulates the cell cycle, probably via the HUSH complex. The HUSH complex is also involved in the silencing of unintegrated retroviral DNA: some part of the retroviral DNA formed immediately after infection remains unintegrated in the host genome and is transcriptionally repressed. May be involved in epithelial differentiation by contributing to epidermal integrity and barrier formation. {ECO:0000250|UniProtKB:Q8NEY8}.

Poids moléculaire: 43.8 kDa

UniProt: Q8K2H1