ERO1L Protein (AA 24-464) (His tag)

N° du produit ABIN3126602

Détail du produit

Antigène: ERO1L (ERO1-Like Protein alpha (ERO1L))

Type de proteíne: Recombinant

Attributs du protein: AA 24-464

Origine: Souris

Source: Cellules d'insectes

Purification/Conjugué: Cette ERO1L protéine est marqué à la His tag.

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS), Crystallization (Crys)

Séquence: EERRPETAAQ RCFCQVSGYL DDCTCDVETI DKFNNYRLFP RLQKLLESDY FRYYKVNLKK PCPFWNDINQ CGRRDCAVKP CHSDEVPDGI KSASYKYSEE ANRIEECEQA ERLGAVDESL SEETQKAVLQ WTKHDDSSDS FCEIDDIQSP DAEYVDLLLN PERYTGYKGP DAWRIWSVIY EENCFKPQTI QRPLASGRGK SKENTFYNWL EGLCVEKRAF YRLISGLHAS INVHLSARYL LQDTWLEKKW GHNVTEFQQR FDGILTEGEG PRRLRNLYFL YLIELRALSK VLPFFERPDF QLFTGNKVQD AENKALLLEI LHEIKSFPLH FDENSFFAGD KNEAHKLKED FRLHFRNISR IMDCVGCFKC RLWGKLQTQG LGTALKILFS EKLIANMPES GPSYEFQLTR QEIVSLFNAF GRISTSVREL ENFRHLLQNV H
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Attributs du produit:

• Made in Germany - from design to production - by highly experienced protein experts.
• Mouse Ero1a Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Protein is endotoxin free.

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Commentaires:

Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: ERO1L (ERO1-Like Protein alpha (ERO1L))

Autre désignation: Ero1a (ERO1L Produits)

Synonymes: ERO1-L Protein, ero1-alpha Protein, ero1lb Protein, ERO1L Protein, ero1l Protein, ERO1-alpha Protein, ERO1A Protein, zgc:63959 Protein, endoplasmic reticulum oxidoreductase alpha L homeolog Protein, endoplasmic reticulum oxidoreductase 1 alpha Protein, endoplasmic reticulum oxidoreductase alpha Protein, ERO1-like (S. cerevisiae) Protein, ero1a.L Protein, ERO1A Protein, ero1a Protein, Ero1l Protein, Ero1a Protein

Sujet: Oxidoreductase involved in disulfide bond formation in the endoplasmic reticulum. Efficiently reoxidizes P4HB/PDI, the enzyme catalyzing protein disulfide formation, in order to allow P4HB to sustain additional rounds of disulfide formation. Following P4HB reoxidation, passes its electrons to molecular oxygen via FAD, leading to the production of reactive oxygen species (ROS) in the cell. Required for the proper folding of immunoglobulins (By similarity). Plays an important role in ER stress-induced, CHOP-dependent apoptosis by activating the inositol 1,4,5-trisphosphate receptor IP3R1. {ECO:0000250, ECO:0000269|PubMed:19752026, ECO:0000269|PubMed:20308425}.

Poids moléculaire: 52.6 kDa Including tag.

UniProt: Q8R180

Pathways: Peptide Hormone Metabolism, ER-Nucleus Signaling, Brown Fat Cell Differentiation