ATG10 Protein (AA 1-215) (Strep Tag)

N° du produit ABIN3126639

Détail du produit

Antigène: ATG10 (Autophagy Related 10 (ATG10))

Type de proteíne: Recombinant

Attributs du protein: AA 1-215

Origine: Souris

Source: Tobacco (Nicotiana tabacum)

Purification/Conjugué: Cette ATG10 protéine est marqué à la Strep Tag.

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Séquence: MEDEFFGEKS FQHYCAEFIR HSQQIGDGWE WRTAKECSDG YMCKTQFRIK NEASTPHVGT PASVLTCLPT EENLELPMDD SEVTRPAAVA EVIKHEYHVL YSCSYQVPVL YFRASFLDGR PLALEDIWEG VHECYKPRLL QGPWDTITQQ EHPILGQPFF VLHPCKTNEF MTAVLKNSQK INRNVNYITS WLSLVGPVVG LNLPLSYAKA TSQSE
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Attributs du produit: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

niveau d'endotoxine: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Commentaires:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: ATG10 (Autophagy Related 10 (ATG10))

Autre désignation: Atg10 (ATG10 Produits)

Synonymes: APG10 Protein, APG10L Protein, pp12616 Protein, Atg10 Protein, ATG10 Protein, MGC123341 Protein, im:7153729 Protein, zgc:123341 Protein, ATATG10 Protein, autophagy 10 Protein, DDBDRAFT_0190076 Protein, DDBDRAFT_0252608 Protein, DDB_0190076 Protein, DDB_0252608 Protein, 5330424L23Rik Protein, 5430428K15Rik Protein, AI852123 Protein, Agp10 Protein, Apg10l Protein, Apg10p Protein, Atg10l Protein, autophagy related 10 Protein, autophagy-related 10 Protein, AuTophaGy (yeast Atg homolog) Protein, ATG10 autophagy related 10 homolog (S. cerevisiae) Protein, autophagocytosis-associated family protein Protein, E2-like conjugating enzyme Protein, hypothetical protein Protein, autophagy protein 10 Protein, autophagy-related protein 10 Protein, ATG10 Protein, Atg10 Protein, atg-10 Protein, atg10 Protein, CAALFM_C302930WA Protein, APG10 Protein

Sujet: Ubiquitin-like-conjugating enzyme ATG10 (EC 2.3.2.-) (Autophagy-related protein 10) (APG10-like) (mAPG10),FUNCTION: E2-like enzyme involved in autophagy. Acts as an E2-like enzyme that catalyzes the conjugation of ATG12 to ATG5. ATG12 conjugation to ATG5 is required for autophagy. Likely serves as an ATG5-recognition molecule. Not involved in ATG12 conjugation to ATG3. Plays a role in adenovirus-mediated cell lysis. {ECO:0000269|PubMed:12890687, ECO:0000269|PubMed:20723759, ECO:0000269|PubMed:21367888}.

Poids moléculaire: 24.6 kDa

UniProt: Q8R1P4

Pathways: ER-Nucleus Signaling, Autophagy