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SP7 Protein (AA 1-428) (His tag)

Crystallography grade SP7 Origine: Souris Hôte: Cellules d'insectes Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
N° du produit ABIN3127212
  • Antigène Voir toutes SP7 Protéines
    SP7 (Sp7 Transcription Factor (SP7))
    Type de proteíne
    Recombinant
    Attributs du protein
    AA 1-428
    Origine
    • 4
    • 2
    Souris
    Source
    • 3
    • 2
    • 1
    Cellules d'insectes
    Purification/Conjugué
    Cette SP7 protéine est marqué à la His tag.
    Application
    Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)
    Séquence
    MASSLLEEEA HYGSSPLAML TAACSKFGGS SPLRDSTTLG KGGTKKPYAD LSAPKTMGDA YPAPFSSTNG LLSPAGSPPA PASGYANDYP PFPHSFPGPT GAQDPGLLVP KGHSSSDCLP SVYTSLDMTH PYGSWYKAGI HAGISPGPGN TPTPWWDMHP GGNWLGGGQG QGDGLQGTLS TGPAQPPLNP QLPTYPSDFA PLNPAPYPAP HLLQPGPQHV LPQDVYKPKA VGNSGQLEGS GAAKPPRGAG TGGSGGYAGS GAGRSTCDCP NCQELERLGA AAAGLRKKPI HSCHIPGCGK VYGKASHLKA HLRWHTGERP FVCNWLFCGK RFTRSDELER HVRTHTREKK FTCLLCSKRF TRSDHLSKHQ RTHGEPGPGP PPSGPKELGE GRSVGEEEAN QPPRSSTSPA PPEKAHGGSP EQSNLLEI
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Attributs du produit
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Mouse Sp7 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Purification
    Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Pureté
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Stérilité
    0.22 μm filtered
    niveau d'endotoxine
    Protein is endotoxin free.
    Classe de qualité
    Crystallography grade
    Top Product
    Discover our top product SP7 Protéine
  • Indications d'application
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    Commentaires

    Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Conseil sur la manipulation
    Avoid repeated freeze-thaw cycles.
    Stock
    -80 °C
    Stockage commentaire
    Store at -80°C.
    Date de péremption
    Unlimited (if stored properly)
  • Antigène
    SP7 (Sp7 Transcription Factor (SP7))
    Autre désignation
    Sp7 (SP7 Produits)
    Synonymes
    BBBG1 Protein, FXY Protein, GBBB1 Protein, MIDIN Protein, OGS1 Protein, OS Protein, OSX Protein, RNF59 Protein, TRIM18 Protein, XPRF Protein, ZNFXY Protein, OI11 Protein, OI12 Protein, osterix Protein, Osx Protein, osx Protein, SP7 Protein, sp7 Protein, 6430578P22Rik Protein, C22 Protein, midline 1 Protein, Sp7 transcription factor Protein, uncharacterized LOC100063836 Protein, Sp7 transcription factor L homeolog Protein, Sp7 transcription factor 7 Protein, MID1 Protein, SP7 Protein, Sp7 Protein, sp7 Protein, LOC100063836 Protein, sp7.L Protein
    Sujet
    Transcriptional activator essential for osteoblast differentiation. Binds to SP1 and EKLF consensus sequences and to other G/C-rich sequences. {ECO:0000269|PubMed:11792318}.
    Poids moléculaire
    45.7 kDa Including tag.
    UniProt
    Q8VI67
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